7nrz
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of malate dehydrogenase from Trypanosoma cruzi== | ==Crystal structure of malate dehydrogenase from Trypanosoma cruzi== | ||
| - | <StructureSection load='7nrz' size='340' side='right'caption='[[7nrz]]' scene=''> | + | <StructureSection load='7nrz' size='340' side='right'caption='[[7nrz]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NRZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7nrz]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi_strain_CL_Brener Trypanosoma cruzi strain CL Brener]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NRZ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nrz OCA], [https://pdbe.org/7nrz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nrz RCSB], [https://www.ebi.ac.uk/pdbsum/7nrz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nrz ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nrz OCA], [https://pdbe.org/7nrz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nrz RCSB], [https://www.ebi.ac.uk/pdbsum/7nrz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nrz ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q4DRD8_TRYCC Q4DRD8_TRYCC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glycosomal malate dehydrogenase from Trypanosoma cruzi (tcgMDH) catalyzes the oxidation/reduction of malate/oxaloacetate, a crucial step of the glycolytic process occurring in the glycosome of the human parasite. Inhibition of tcgMDH is considered a druggable trait for the development of trypanocidal drugs. Sequence comparison of MDHs from different organisms revealed a distinct insertion of a prolin rich 9-mer (62-KLPPVPRDP-70) in tcgMDH as compared to other eukaryotic MDHs. Crystal structure of tcgMDH is solved here at 2.6 A resolution with Rwork/Rfree values of 0.206/0.216. The tcgMDH forms homo-dimer with the solvation free energy (DeltaG(o)) gain of -9.77 kcal/mol. The dimeric form is also confirmed in solution by biochemical assays, chemical-crosslinking and dynamic light scattering. The inserted 9-mer adopts a structure of a solvent accessible loop in the vicinity of NAD(+) binding site. The distinct sequence and structural feature of tcgMDH, revealed in the present report, provides an anchor point for the development of inhibitors specific for tcgMDH, possible trypanocidal agents of the future. | ||
| + | |||
| + | Distinct sequence and structural feature of trypanosoma malate dehydrogenase.,Sonani RR, Kurpiewska K, Lewinski K, Dubin G Biochem Biophys Res Commun. 2021 Jun 11;557:288-293. doi:, 10.1016/j.bbrc.2021.04.033. Epub 2021 Apr 21. PMID:33894416<ref>PMID:33894416</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7nrz" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Malate Dehydrogenase 3D structures|Malate Dehydrogenase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Trypanosoma cruzi strain CL Brener]] | ||
[[Category: Dubin G]] | [[Category: Dubin G]] | ||
[[Category: Kurpiewska K]] | [[Category: Kurpiewska K]] | ||
[[Category: Lewinski K]] | [[Category: Lewinski K]] | ||
[[Category: Sonani RR]] | [[Category: Sonani RR]] | ||
Current revision
Crystal structure of malate dehydrogenase from Trypanosoma cruzi
| |||||||||||
