7o0e
From Proteopedia
(Difference between revisions)
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==Crystal structure of GH30 (mutant E188A) complexed with aldotriuronic acid from Thermothelomyces thermophila.== | ==Crystal structure of GH30 (mutant E188A) complexed with aldotriuronic acid from Thermothelomyces thermophila.== | ||
| - | <StructureSection load='7o0e' size='340' side='right'caption='[[7o0e]]' scene=''> | + | <StructureSection load='7o0e' size='340' side='right'caption='[[7o0e]], [[Resolution|resolution]] 1.85Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O0E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7o0e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothelomyces_thermophilus_ATCC_42464 Thermothelomyces thermophilus ATCC 42464]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O0E FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o0e OCA], [https://pdbe.org/7o0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o0e RCSB], [https://www.ebi.ac.uk/pdbsum/7o0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o0e ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GCV:4-O-METHYL-ALPHA-D-GLUCURONIC+ACID'>GCV</scene>, <scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o0e OCA], [https://pdbe.org/7o0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o0e RCSB], [https://www.ebi.ac.uk/pdbsum/7o0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o0e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/XY30A_MYCTT XY30A_MYCTT] Xylanase exhibiting endo- and exo-xylanase activity (PubMed:31110561). Shows the highest activity toward beechwood glucuronoxylan, which consists of a beta-1,4-linked xylose backbone decorated with the methylated form of D-glucuronic acid (MeGlcA) attached directly to the main chain at xylose C2 (PubMed:31110561). Acts also against wheat arabinoxylan, a xylan without MeGlcA substituents along the main chain, but the xylanase activity is about two orders of magnitude lower than that achieved in the case of beechwood xylan (PubMed:31110561). Shows no activity against carob galactomannan, konjac glucomannan, or barley beta-glucan (PubMed:31110561). The recombinant xylanase also exhibits an exo-activity by releasing processively disaccharide units from the non-reducing end of linear and decorated xylooligosaccharides (XOS) (PubMed:31110561).<ref>PMID:31110561</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Thermothelomyces thermophilus ATCC 42464]] | ||
[[Category: Dimarogona M]] | [[Category: Dimarogona M]] | ||
[[Category: Feiler C]] | [[Category: Feiler C]] | ||
Revision as of 12:42, 1 February 2024
Crystal structure of GH30 (mutant E188A) complexed with aldotriuronic acid from Thermothelomyces thermophila.
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