7o62
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of a 2`-deoxyribosyltransferase from the psychrophilic bacterium Desulfotalea psychrophila.== | ==Crystal structure of a 2`-deoxyribosyltransferase from the psychrophilic bacterium Desulfotalea psychrophila.== | ||
| - | <StructureSection load='7o62' size='340' side='right'caption='[[7o62]]' scene=''> | + | <StructureSection load='7o62' size='340' side='right'caption='[[7o62]], [[Resolution|resolution]] 2.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O62 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7o62]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfotalea_psychrophila_LSv54 Desulfotalea psychrophila LSv54]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O62 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o62 OCA], [https://pdbe.org/7o62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o62 RCSB], [https://www.ebi.ac.uk/pdbsum/7o62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o62 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o62 OCA], [https://pdbe.org/7o62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o62 RCSB], [https://www.ebi.ac.uk/pdbsum/7o62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o62 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q6AQB0_DESPS Q6AQB0_DESPS] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nucleoside 2'-deoxyribosyltransferases (NDTs) catalyze the cleavage of glycosidic bonds of 2'-deoxynucleosides and the following transfer of the 2'-deoxyribose moiety to acceptor nucleobases. Here, we report the crystal structures and biochemical properties of the first tetrameric NDTs: the type I NDT from the mesophilic bacterium Enterococcus faecalis V583 (EfPDT) and the type II NDT from the bacterium Desulfotalea psychrophila (DpNDT), the first psychrophilic NDT. This novel structural and biochemical data permitted an exhaustive comparative analysis aimed to shed light into the basis of the high global stability of the psychrophilic DpNDT, which has a higher melting temperature than EfPDT (58.5 degrees C versus 54.4 degrees C) or other mesophilic NDTs. DpNDT possesses a combination of unusual structural motifs not present neither in EfPDT nor any other NDT that most probably contribute to its global stability, in particular, a large aliphatic isoleucine-leucine-valine (ILV) bundle accompanied by a vicinal disulfide bridge and also an intersubunit disulfide bridge, the first described for an NDT. The functional and structural features of DpNDT do not fit the standard features of psychrophilic enzymes, which lead us to consider the implication of (sub)cellular levels together with the protein level in the adaptation of enzymatic activity to low temperatures. | ||
| + | |||
| + | Biochemical and structural studies of two tetrameric nucleoside 2'-deoxyribosyltransferases from psychrophilic and mesophilic bacteria: Insights into cold-adaptation.,Fernandez-Lucas J, Acebron I, Wu RY, Alfaro Y, Acosta J, Kaminski PA, Arroyo M, Joachimiak A, Nocek BP, De la Mata I, Mancheno JM Int J Biol Macromol. 2021 Oct 5;192:138-150. doi: 10.1016/j.ijbiomac.2021.09.164. PMID:34624379<ref>PMID:34624379</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7o62" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Desulfotalea psychrophila LSv54]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Mancheno JM]] | [[Category: Mancheno JM]] | ||
Revision as of 12:45, 1 February 2024
Crystal structure of a 2`-deoxyribosyltransferase from the psychrophilic bacterium Desulfotalea psychrophila.
| |||||||||||
