7p3t
From Proteopedia
(Difference between revisions)
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==Transaminase of gamma-proteobacterium== | ==Transaminase of gamma-proteobacterium== | ||
| - | <StructureSection load='7p3t' size='340' side='right'caption='[[7p3t]]' scene=''> | + | <StructureSection load='7p3t' size='340' side='right'caption='[[7p3t]], [[Resolution|resolution]] 1.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P3T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7p3t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Luminiphilus_syltensis_NOR5-1B Luminiphilus syltensis NOR5-1B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P3T FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p3t OCA], [https://pdbe.org/7p3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p3t RCSB], [https://www.ebi.ac.uk/pdbsum/7p3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p3t ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p3t OCA], [https://pdbe.org/7p3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p3t RCSB], [https://www.ebi.ac.uk/pdbsum/7p3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p3t ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/B8KQT8_9GAMM B8KQT8_9GAMM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Despite the plethora of information on (S)-selective amine transaminases, the (R)-selective ones are still not well-studied; only a few structures are known to date, and their substrate scope is limited, apart from a few stellar works in the field. Herein, the structure of Luminiphilus syltensis (R)-selective amine transaminase is elucidated to facilitate engineering towards variants active on bulkier substrates. The V37A variant exhibited increased activity towards 1-phenylpropylamine and to activity against 1-butylamine. In contrast, the S248 and T249 positions, located on the beta-turn in the P-pocket, seem crucial for maintaining the activity of the enzyme. | ||
| + | |||
| + | Rational engineering of Luminiphilus syltensis (R)-selective amine transaminase for the acceptance of bulky substrates.,Konia E, Chatzicharalampous K, Drakonaki A, Muenke C, Ermler U, Tsiotis G, Pavlidis IV Chem Commun (Camb). 2021 Dec 3;57(96):12948-12951. doi: 10.1039/d1cc04664k. PMID:34806715<ref>PMID:34806715</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7p3t" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Aminotransferase 3D structures|Aminotransferase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Luminiphilus syltensis NOR5-1B]] | ||
[[Category: Ermler U]] | [[Category: Ermler U]] | ||
Current revision
Transaminase of gamma-proteobacterium
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