7pnh
From Proteopedia
(Difference between revisions)
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==X-ray structure of the adduct formed upon reaction of Pt(II) complex 2c with lysozyme== | ==X-ray structure of the adduct formed upon reaction of Pt(II) complex 2c with lysozyme== | ||
| - | <StructureSection load='7pnh' size='340' side='right'caption='[[7pnh]]' scene=''> | + | <StructureSection load='7pnh' size='340' side='right'caption='[[7pnh]], [[Resolution|resolution]] 1.22Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PNH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7pnh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PNH FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pnh OCA], [https://pdbe.org/7pnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pnh RCSB], [https://www.ebi.ac.uk/pdbsum/7pnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pnh ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.22Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pnh OCA], [https://pdbe.org/7pnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pnh RCSB], [https://www.ebi.ac.uk/pdbsum/7pnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pnh ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Three novel platinum(II) complexes bearing N-heterocyclic ligands, i.e., Pt2c, Pt-IV and Pt-VIII, were previously prepared and characterized. They manifested promising in vitro anticancer properties associated with non-conventional modes of action. To gain further mechanistic insight, we have explored here the reactions of these Pt compounds with a few model proteins, i.e., hen egg white lysozyme (HEWL), bovine pancreatic ribonuclease (RNase A), horse heart cytochrome c (Cyt-c) and human serum albumin (HSA), primarily through ESI MS analysis. Characteristic and variegate patterns of reactivity were highlighted in the various cases that appear to depend both on the nature of the Pt complex and of the interacting protein. The protein-bound Pt fragments were identified. In the case of the complex Pt2c, the adducts formed upon reaction with HEWL and RNase A were further characterized by solving the respective crystal structures: this allowed us to determine the exact location of the various Pt binding sites. The implications of the obtained results are discussed in relation to the possible mechanisms of action of these innovative anticancer Pt complexes. | ||
| + | |||
| + | Reactions with Proteins of Three Novel Anticancer Platinum(II) Complexes Bearing N-Heterocyclic Ligands.,Sacco F, Tarchi M, Ferraro G, Merlino A, Facchetti G, Rimoldi I, Messori L, Massai L Int J Mol Sci. 2021 Sep 29;22(19). pii: ijms221910551. doi:, 10.3390/ijms221910551. PMID:34638887<ref>PMID:34638887</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7pnh" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Gallus gallus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Ferraro G]] | [[Category: Ferraro G]] | ||
[[Category: Merlino A]] | [[Category: Merlino A]] | ||
Revision as of 13:06, 1 February 2024
X-ray structure of the adduct formed upon reaction of Pt(II) complex 2c with lysozyme
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