1pxd
From Proteopedia
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'''Crystal structure of the complex of jacalin with meso-tetrasulphonatophenylporphyrin.''' | '''Crystal structure of the complex of jacalin with meso-tetrasulphonatophenylporphyrin.''' | ||
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[[Category: Salunke, D M.]] | [[Category: Salunke, D M.]] | ||
[[Category: Swamy, M J.]] | [[Category: Swamy, M J.]] | ||
| - | [[Category: | + | [[Category: Lectin]] |
| - | [[Category: | + | [[Category: Porphyrin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:35:54 2008'' | |
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Revision as of 02:35, 3 May 2008
Crystal structure of the complex of jacalin with meso-tetrasulphonatophenylporphyrin.
Overview
The crystal structure of the complex of meso-tetrasulfonatophenylporphyrin (H(2)TPPS) with jack fruit (Artocarpus integriflora) agglutinin (jacalin) has been determined at 1.8 A resolution. A porphyrin pair is sandwiched between two symmetry-related jacalin monomers in the crystal, leading to a cross-linking network of protein molecules. Apart from the stacking interactions, H(2)TPPS also forms hydrogen bonds, some involving water bridges, with jacalin at the carbohydrate-binding site. The residues that are involved in rendering galactopyranoside specificity to jacalin undergo conformational adjustments in order to accommodate the H(2)TPPS molecule. The water molecules at the carbohydrate-binding site of jacalin cement the jacalin-porphyrin interactions, optimizing their complementarity. Interactions of porphyrin with jacalin are relatively weak compared with those observed between galactopyranoside and jacalin, perhaps because the former largely involves water-mediated hydrogen bonds. While H(2)TPPS binds to jacalin at the carbohydrate-binding site as in the case of ConA, its mode of interaction with jacalin is very different. H(2)TPPS does not enter the carbohydrate-binding cavity of jacalin. Instead, it sits over the binding site. While the porphyrin binding is mediated by replicating the hydrogen-bonding network of mannopyranoside through the sulfonate atoms in the case of ConA, the plasticity associated with the carbohydrate-binding site accommodates the pluripotent porphyrin molecule in the case of jacalin through an entirely different set of interactions.
About this Structure
1PXD is a Protein complex structure of sequences from Artocarpus integer. Full crystallographic information is available from OCA.
Reference
Porphyrin binding to jacalin is facilitated by the inherent plasticity of the carbohydrate-binding site: novel mode of lectin-ligand interaction., Goel M, Anuradha P, Kaur KJ, Maiya BG, Swamy MJ, Salunke DM, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):281-8. Epub 2004, Jan 23. PMID:14747704 Page seeded by OCA on Sat May 3 05:35:54 2008
