8a4c
From Proteopedia
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| - | ==== | + | ==Structure of human Rep15:Rab3B complex.== |
| - | <StructureSection load='8a4c' size='340' side='right'caption='[[8a4c]]' scene=''> | + | <StructureSection load='8a4c' size='340' side='right'caption='[[8a4c]], [[Resolution|resolution]] 2.75Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[8a4c]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A4C FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a4c OCA], [https://pdbe.org/8a4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a4c RCSB], [https://www.ebi.ac.uk/pdbsum/8a4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a4c ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a4c OCA], [https://pdbe.org/8a4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a4c RCSB], [https://www.ebi.ac.uk/pdbsum/8a4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a4c ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/REP15_HUMAN REP15_HUMAN] Regulates transferrin receptor recycling from the endocytic recycling compartment.<ref>PMID:16195351</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In their GTP-bound (active) form, Rab proteins interact with effector proteins that control downstream signaling. One such Rab15 effector is Rep15, which is known to have a role in receptor recycling from the endocytic recycling compartment but otherwise remains poorly characterized. Here, we report the characterization of the Rep15:Rab15 interaction and identification of Rab3 paralogs and Rab34 as Rep15 interacting partners from a yeast two-hybrid assay. Biochemical validation of the interactions is presented and crystal structures of the Rep15:Rab3B and Rep15:Rab3C complexes provide additional mechanistic insight. We find that Rep15 adopts a globular structure that is distinct from other reported Rab15, Rab3 and Rab34 effectors. Structure-based mutagenesis experiments explain the Rep15:Rab interaction specificity. Rep15 depletion in U138MG glioblastoma cells impairs cell proliferation, cell migration and receptor recycling, underscoring the need for further clarification of the role of Rep15 in cancer. | ||
| + | |||
| + | Rep15 interacts with several Rab GTPases and has a distinct fold for a Rab effector.,Rai A, Singh AK, Bleimling N, Posern G, Vetter IR, Goody RS Nat Commun. 2022 Jul 23;13(1):4262. doi: 10.1038/s41467-022-31831-1. PMID:35871249<ref>PMID:35871249</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 8a4c" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Ras-related protein Rab 3D structures|Ras-related protein Rab 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Goody RS]] |
| + | [[Category: Rai A]] | ||
| + | [[Category: Vetter IR]] | ||
Current revision
Structure of human Rep15:Rab3B complex.
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