1dt0

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<StructureSection load='1dt0' size='340' side='right'caption='[[1dt0]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='1dt0' size='340' side='right'caption='[[1dt0]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1dt0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DT0 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1dt0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DT0 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ar4|1ar4]], [[1ids|1ids]], [[1isb|1isb]], [[1isc|1isc]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dt0 OCA], [https://pdbe.org/1dt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dt0 RCSB], [https://www.ebi.ac.uk/pdbsum/1dt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dt0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dt0 OCA], [https://pdbe.org/1dt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dt0 RCSB], [https://www.ebi.ac.uk/pdbsum/1dt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dt0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/SODF_PSEPU SODF_PSEPU]] Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
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[https://www.uniprot.org/uniprot/SODF_PSEPU SODF_PSEPU] Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dt0 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dt0 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
 
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== Publication Abstract from PubMed ==
 
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The gene encoding the iron-dependent superoxide dismutase from Pseudomonas ovalis was cloned from a genomic library and sequenced. The ORF differs from the previously published protein sequence, which was used for the original structure determination, at 16 positions. The differences include three additional inserted residues, one deleted residue and 12 point substitutions. The gene was subcloned and the recombinant protein overexpressed, purified and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 A resolution.
 
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Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis.,Bond CJ, Huang J, Hajduk R, Flick KE, Heath PJ, Stoddard BL Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1359-66. PMID:11053832<ref>PMID:11053832</ref>
 
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
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</div>
 
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<div class="pdbe-citations 1dt0" style="background-color:#fffaf0;"></div>
 
==See Also==
==See Also==
*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus fluorescens putidus flugge 1886]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Superoxide dismutase]]
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[[Category: Pseudomonas putida]]
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[[Category: Bond, C J]]
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[[Category: Bond CJ]]
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[[Category: Flick, K]]
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[[Category: Flick K]]
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[[Category: Hajduk, R]]
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[[Category: Hajduk R]]
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[[Category: Heath, P]]
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[[Category: Heath P]]
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[[Category: Huang, J]]
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[[Category: Huang J]]
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[[Category: Stoddard, B L]]
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[[Category: Stoddard BL]]
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[[Category: Oxidoreductase]]
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[[Category: Pseudomonas ovali]]
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Current revision

CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS

PDB ID 1dt0

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