1ryh
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(New page: 200px<br /> <applet load="1ryh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ryh, resolution 1.75Å" /> '''Alternative Splicin...)
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Revision as of 17:01, 12 November 2007
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Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase
Overview
Rac1b was recently identified in malignant colorectal tumors as an, alternative splice variant of Rac1 containing a 19-amino acid insertion, next to the switch II region. The structures of Rac1b in the GDP- and the, GppNHp-bound forms, determined at a resolution of 1.75 A, reveal that the, insertion induces an open switch I conformation and a highly mobile switch, II. As a consequence, Rac1b has an accelerated GEF-independent GDP/GTP, exchange and an impaired GTP hydrolysis, which is restored partially by, GTPase-activating proteins. Interestingly, Rac1b is able to bind the, GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent, manner, suggesting that the insertion does not completely abolish effector, interaction. The presented study provides insights into the structural and, biochemical mechanism of a self-activating GTPase.
About this Structure
1RYH is a Single protein structure of sequence from Homo sapiens with MG and GNP as ligands. Active as Small monomeric GTPase, with EC number 3.6.5.2 Full crystallographic information is available from OCA.
Reference
Alternative splicing of Rac1 generates Rac1b, a self-activating GTPase., Fiegen D, Haeusler LC, Blumenstein L, Herbrand U, Dvorsky R, Vetter IR, Ahmadian MR, J Biol Chem. 2004 Feb 6;279(6):4743-9. Epub 2003 Nov 18. PMID:14625275
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