1js2
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
<StructureSection load='1js2' size='340' side='right'caption='[[1js2]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='1js2' size='340' side='right'caption='[[1js2]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1js2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1js2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Allochromatium_vinosum Allochromatium vinosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JS2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1js2 OCA], [https://pdbe.org/1js2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1js2 RCSB], [https://www.ebi.ac.uk/pdbsum/1js2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1js2 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1js2 OCA], [https://pdbe.org/1js2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1js2 RCSB], [https://www.ebi.ac.uk/pdbsum/1js2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1js2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/HIP_ALLVD HIP_ALLVD] Specific class of high-redox-potential 4Fe-4S ferredoxins. Functions in anaerobic electron transport in most purple and in some other photosynthetic bacteria and in at least one genus (Paracoccus) of halophilic, denitrifying bacteria. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 19: | Line 20: | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1js2 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1js2 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The crystal structure of Chromatium vinosum C77S HiPIP has been determined and is compared with that of wild type. This is the first reported crystal structure of a Ser ligated [4Fe-4S] cluster and reveals a 0.11 A shortening of the Fe-O bond (relative to Fe-S), but only minor structural alterations of the overall tertiary structure. Coordination changes are corroborated by resonance Raman spectroscopy. Comparison of the crystal and solution structures for HiPIPs identifies Phe48 as the main controller of solvent access to the Fe-S cluster; however, there is no significant change in cluster solvation of the C77S mutant relative to WT HiPIP. Ser ligation ultimately results in decreased cluster stability due to increased sensitivity to proton mediated degradation. | ||
| - | |||
| - | Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster.,Mansy SS, Xiong Y, Hemann C, Hille R, Sundaralingam M, Cowan JA Biochemistry. 2002 Jan 29;41(4):1195-201. PMID:11802718<ref>PMID:11802718</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1js2" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Allochromatium vinosum]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Cowan | + | [[Category: Cowan JA]] |
| - | [[Category: Hemann | + | [[Category: Hemann C]] |
| - | [[Category: Hille | + | [[Category: Hille R]] |
| - | [[Category: Mansy | + | [[Category: Mansy SS]] |
| - | [[Category: Sundaralingam | + | [[Category: Sundaralingam M]] |
| - | [[Category: Xiong | + | [[Category: Xiong Y]] |
| - | + | ||
Current revision
Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S] cluster
| |||||||||||
