8b28
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[8b28]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vitis_vinifera Vitis vinifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B28 FirstGlance]. <br> | <table><tr><td colspan='2'>[[8b28]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vitis_vinifera Vitis vinifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B28 FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b28 OCA], [https://pdbe.org/8b28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b28 RCSB], [https://www.ebi.ac.uk/pdbsum/8b28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b28 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b28 OCA], [https://pdbe.org/8b28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b28 RCSB], [https://www.ebi.ac.uk/pdbsum/8b28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b28 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/G3F1Q6_VITVI G3F1Q6_VITVI] | [https://www.uniprot.org/uniprot/G3F1Q6_VITVI G3F1Q6_VITVI] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Enhanced Disease Susceptibility 1 (EDS1), a key component of microbe-triggered immunity and effector-triggered immunity in most higher plants, forms functional heterodimeric complexes with its homologs Phytoalexin Deficient 4 (PAD4) or Senescence-associated Gene 101 (SAG101). Here, the crystal structure of VvEDS1(Nterm) , the N-terminal domain of EDS1 from Vitis vinifera, is reported, representing the first structure of an EDS1 entity beyond the model plant Arabidopsis thaliana. VvEDS1(Nterm) has an alpha/beta-hydrolase fold, is similar to the N-terminal domain of A. thaliana EDS1 and forms stable homodimers in solution as well as in crystals. These VvEDS1(Nterm) homodimers are spatially incompatible with heterodimers with PAD4 or SAG101, they explain why VvEDS1(Nterm) does not interact with V. vinifera PAD4 according to gel filtration, and they serve as a guide to develop a plausible, albeit experimentally not verified model of full-length EDS1. VvEDS1(Nterm) is a splicing variant comprising two of three exons of the VvEDS1 gene. It originates from a naturally occurring mRNA, in which the first of two introns was removed while the second one containing a stop codon close to the exon/intron border was retained. This is a potential case of intron retention and the first report of this phenomenon in the context of EDS1. Its biological significance has not yet been clarified, nor has the question if a VvEDS1(Nterm) protein with a specific function can occur under physiological conditions. | ||
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| - | A splicing variant of EDS1 from Vitis vinifera forms homodimers but no heterodimers with PAD4.,Voss M, Cseke LJ, Gassmann W, Niefind K Protein Sci. 2023 Apr;32(4):e4624. doi: 10.1002/pro.4624. PMID:36917448<ref>PMID:36917448</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 8b28" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Structure of an intron-retention variant of the plant immune signalling protein EDS1 from Vitis vinifera
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