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8b2w
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[8b2w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B2W FirstGlance]. <br> | <table><tr><td colspan='2'>[[8b2w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8B2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8B2W FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AIX:(2R,4S)-2-[(1R)-1-{[(2R)-2-AMINO-2-PHENYLACETYL]AMINO}-2-OXOETHYL]-5,5-DIMETHYL-1,3-THIAZOLIDINE-4-CARBOXYLIC+ACID'>AIX</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AIX:(2R,4S)-2-[(1R)-1-{[(2R)-2-AMINO-2-PHENYLACETYL]AMINO}-2-OXOETHYL]-5,5-DIMETHYL-1,3-THIAZOLIDINE-4-CARBOXYLIC+ACID'>AIX</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b2w OCA], [https://pdbe.org/8b2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b2w RCSB], [https://www.ebi.ac.uk/pdbsum/8b2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b2w ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8b2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8b2w OCA], [https://pdbe.org/8b2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8b2w RCSB], [https://www.ebi.ac.uk/pdbsum/8b2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8b2w ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/D2D9A0_KLEPN D2D9A0_KLEPN] | [https://www.uniprot.org/uniprot/D2D9A0_KLEPN D2D9A0_KLEPN] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | We introduce the spitrobot, a protein crystal plunger, enabling reaction quenching via cryo-trapping with a time-resolution in the millisecond range. Protein crystals are mounted on canonical micromeshes on an electropneumatic piston, where the crystals are kept in a humidity and temperature-controlled environment, then reactions are initiated via the liquid application method (LAMA) and plunging into liquid nitrogen is initiated after an electronically set delay time to cryo-trap intermediate states. High-magnification images are automatically recorded before and after droplet deposition, prior to plunging. The SPINE-standard sample holder is directly plunged into a storage puck, enabling compatibility with high-throughput infrastructure. Here we demonstrate binding of glucose and 2,3-butanediol in microcrystals of xylose isomerase, and of avibactam and ampicillin in microcrystals of the extended spectrum beta-lactamase CTX-M-14. We also trap reaction intermediates and conformational changes in macroscopic crystals of tryptophan synthase to demonstrate that the spitrobot enables insight into catalytic events. | ||
| - | |||
| - | Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography.,Mehrabi P, Sung S, von Stetten D, Prester A, Hatton CE, Kleine-Dopke S, Berkes A, Gore G, Leimkohl JP, Schikora H, Kollewe M, Rohde H, Wilmanns M, Tellkamp F, Schulz EC Nat Commun. 2023 Apr 25;14(1):2365. doi: 10.1038/s41467-023-37834-w. PMID:37185266<ref>PMID:37185266</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 8b2w" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Millisecond cryo-trapping by the spitrobot crystal plunger, CTX-M-14 E166A, Ampicillin, 500 MS
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Categories: Klebsiella pneumoniae | Large Structures | Berkes A | Gore G | Hatton CE | Kleine-Doepke S | Kollewe M | Leimkohl JP | Mehrabi P | Prester A | Rohde H | Schikora H | Schulz EC | Sung S | Tellkamp F | Wilmanns M | Von Stetten D
