3j95

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<SX load='3j95' size='340' side='right' viewer='molstar' caption='[[3j95]], [[Resolution|resolution]] 7.60&Aring;' scene=''>
<SX load='3j95' size='340' side='right' viewer='molstar' caption='[[3j95]], [[Resolution|resolution]] 7.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3j95]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Cho_cell_lines Cho cell lines]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J95 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3j95]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J95 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.601&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3j94|3j94]], [[3j96|3j96]], [[3j97|3j97]], [[3j98|3j98]], [[3j99|3j99]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NSF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10029 CHO cell lines])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Vesicle-fusing_ATPase Vesicle-fusing ATPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.6 3.6.4.6] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j95 OCA], [https://pdbe.org/3j95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j95 RCSB], [https://www.ebi.ac.uk/pdbsum/3j95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j95 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j95 OCA], [https://pdbe.org/3j95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j95 RCSB], [https://www.ebi.ac.uk/pdbsum/3j95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j95 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR]] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.
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[https://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Evolutionarily conserved SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) proteins form a complex that drives membrane fusion in eukaryotes. The ATPase NSF (N-ethylmaleimide sensitive factor), together with SNAPs (soluble NSF attachment protein), disassembles the SNARE complex into its protein components, making individual SNAREs available for subsequent rounds of fusion. Here we report structures of ATP- and ADP-bound NSF, and the NSF/SNAP/SNARE (20S) supercomplex determined by single-particle electron cryomicroscopy at near-atomic to sub-nanometre resolution without imposing symmetry. Large, potentially force-generating, conformational differences exist between ATP- and ADP-bound NSF. The 20S supercomplex exhibits broken symmetry, transitioning from six-fold symmetry of the NSF ATPase domains to pseudo four-fold symmetry of the SNARE complex. SNAPs interact with the SNARE complex with an opposite structural twist, suggesting an unwinding mechanism. The interfaces between NSF, SNAPs, and SNAREs exhibit characteristic electrostatic patterns, suggesting how one NSF/SNAP species can act on many different SNARE complexes.
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Mechanistic insights into the recycling machine of the SNARE complex.,Zhao M, Wu S, Zhou Q, Vivona S, Cipriano DJ, Cheng Y, Brunger AT Nature. 2015 Jan 12. doi: 10.1038/nature14148. PMID:25581794<ref>PMID:25581794</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3j95" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</SX>
</SX>
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[[Category: Cho cell lines]]
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[[Category: Cricetulus griseus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Vesicle-fusing ATPase]]
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[[Category: Brunger AT]]
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[[Category: Brunger, A T]]
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[[Category: Cheng Y]]
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[[Category: Cheng, Y]]
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[[Category: Wu S]]
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[[Category: Wu, S]]
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[[Category: Zhao M]]
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[[Category: Zhao, M]]
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[[Category: Atpases associated with diverse cellular activity]]
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[[Category: Hydrolase]]
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Current revision

Structure of ADP-bound N-ethylmaleimide sensitive factor determined by single particle cryoelectron microscopy

3j95, resolution 7.60Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

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