3ja6

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Current revision (08:41, 7 February 2024) (edit) (undo)
 
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<SX load='3ja6' size='340' side='right' viewer='molstar' caption='[[3ja6]], [[Resolution|resolution]] 12.70&Aring;' scene=''>
<SX load='3ja6' size='340' side='right' viewer='molstar' caption='[[3ja6]], [[Resolution|resolution]] 12.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ja6]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JA6 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ja6]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JA6 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ja6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja6 OCA], [https://pdbe.org/3ja6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ja6 RCSB], [https://www.ebi.ac.uk/pdbsum/3ja6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ja6 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ja6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja6 OCA], [https://pdbe.org/3ja6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ja6 RCSB], [https://www.ebi.ac.uk/pdbsum/3ja6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ja6 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/CHEW_THEMA CHEW_THEMA] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors (By similarity).
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Chemotactic responses in bacteria require large, highly ordered arrays of sensory proteins to mediate the signal transduction that ultimately controls cell motility. A mechanistic understanding of the molecular events underlying signaling, however, has been hampered by the lack of a high-resolution structural description of the extended array. Here, we report a novel reconstitution of the array, involving the receptor signaling domain, histidine kinase CheA, and adaptor protein CheW, as well as a density map of the core-signaling unit at 11.3 A resolution, obtained by cryo-electron tomography and sub-tomogram averaging. Extracting key structural constraints from our density map, we computationally construct and refine an atomic model of the core array structure, exposing novel interfaces between the component proteins. Using all-atom molecular dynamics simulations, we further reveal a distinctive conformational change in CheA. Mutagenesis and chemical cross-linking experiments confirm the importance of the conformational dynamics of CheA for chemotactic function.
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CryoEM and computer simulations reveal a novel kinase conformational switch in bacterial chemotaxis signaling.,Cassidy CK, Himes BA, Alvarez FJ, Ma J, Zhao G, Perilla JR, Schulten K, Zhang P Elife. 2015 Nov 19;4. pii: e08419. doi: 10.7554/eLife.08419. PMID:26583751<ref>PMID:26583751</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ja6" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
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[[Category: Bacillus coli migula 1895]]
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[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Alvarez, F J]]
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[[Category: Alvarez FJ]]
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[[Category: Cassidy, C K]]
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[[Category: Cassidy CK]]
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[[Category: Himes, B A]]
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[[Category: Himes BA]]
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[[Category: Ma, J]]
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[[Category: Ma J]]
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[[Category: Perilla, J R]]
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[[Category: Perilla JR]]
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[[Category: Schulten, K]]
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[[Category: Schulten K]]
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[[Category: Zhang, P]]
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[[Category: Zhang P]]
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[[Category: Zhao, G]]
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[[Category: Zhao G]]
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[[Category: Adaptor protein]]
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[[Category: Bacterial chemotaxis]]
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[[Category: Chemoreceptor]]
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[[Category: Core-signaling unit]]
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[[Category: Histidine kinase]]
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[[Category: Signaling protein]]
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Current revision

Cryo-electron Tomography and All-atom Molecular Dynamics Simulations Reveal a Novel Kinase Conformational Switch in Bacterial Chemotaxis Signaling

3ja6, resolution 12.70Å

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