3ja7

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Current revision (08:41, 7 February 2024) (edit) (undo)
 
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<SX load='3ja7' size='340' side='right' viewer='molstar' caption='[[3ja7]], [[Resolution|resolution]] 3.63&Aring;' scene=''>
<SX load='3ja7' size='340' side='right' viewer='molstar' caption='[[3ja7]], [[Resolution|resolution]] 3.63&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ja7]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JA7 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ja7]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JA7 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">20 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.63&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ja7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja7 OCA], [https://pdbe.org/3ja7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ja7 RCSB], [https://www.ebi.ac.uk/pdbsum/3ja7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ja7 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ja7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ja7 OCA], [https://pdbe.org/3ja7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ja7 RCSB], [https://www.ebi.ac.uk/pdbsum/3ja7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ja7 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/PORTL_BPT4 PORTL_BPT4]] Forms the portal vertex of the capsid (PubMed:2685327, PubMed:24126213). This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel. Binds to the terminase subunits to form the packaging machine. Attaches to the host inner membrane most likely through interaction with host yidC and forms together with chaperone gp40 an initiator complex to form the prohead.<ref>PMID:24126213</ref> <ref>PMID:2685327</ref>
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[https://www.uniprot.org/uniprot/PORTL_BPT4 PORTL_BPT4] Forms the portal vertex of the capsid (PubMed:2685327, PubMed:24126213). This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection. The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel. Binds to the terminase subunits to form the packaging machine. Attaches to the host inner membrane most likely through interaction with host yidC and forms together with chaperone gp40 an initiator complex to form the prohead.<ref>PMID:24126213</ref> <ref>PMID:2685327</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure and assembly of bacteriophage T4 has been extensively studied. However, the detailed structure of the portal protein remained unknown. Here we report the structure of the bacteriophage T4 portal assembly, gene product 20 (gp20), determined by cryo-electron microscopy (cryo-EM) to 3.6 A resolution. In addition, analysis of a 10 A resolution cryo-EM map of an empty prolate T4 head shows how the dodecameric portal assembly interacts with the capsid protein gp23 at the special pentameric vertex. The gp20 structure also verifies that the portal assembly is required for initiating head assembly, for attachment of the packaging motor, and for participation in DNA packaging. Comparison of the Myoviridae T4 portal structure with the known portal structures of phi29, SPP1 and P22, representing Podo- and Siphoviridae, shows that the portal structure probably dates back to a time when self-replicating microorganisms were being established on Earth.
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Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution.,Sun L, Zhang X, Gao S, Rao PA, Padilla-Sanchez V, Chen Z, Sun S, Xiang Y, Subramaniam S, Rao VB, Rossmann MG Nat Commun. 2015 Jul 6;6:7548. doi: 10.1038/ncomms8548. PMID:26144253<ref>PMID:26144253</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ja7" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Portal protein|Portal protein]]
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*[[Portal protein 3D structures|Portal protein 3D structures]]
== References ==
== References ==
<references/>
<references/>
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__TOC__
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[[Category: Bpt4]]
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[[Category: Escherichia virus T4]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Chen, Z]]
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[[Category: Chen Z]]
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[[Category: Gao, S]]
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[[Category: Gao S]]
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[[Category: Padilla-Sanchez, V]]
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[[Category: Padilla-Sanchez V]]
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[[Category: Rao, P A]]
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[[Category: Rao PA]]
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[[Category: Rao, V B]]
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[[Category: Rao VB]]
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[[Category: Rossmann, M G]]
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[[Category: Rossmann MG]]
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[[Category: Subramaniam, S]]
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[[Category: Subramaniam S]]
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[[Category: Sun, L]]
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[[Category: Sun L]]
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[[Category: Sun, S]]
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[[Category: Sun S]]
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[[Category: Xiang, Y]]
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[[Category: Xiang Y]]
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[[Category: Zhang, X]]
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[[Category: Zhang X]]
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[[Category: Viral protein]]
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Current revision

Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution

3ja7, resolution 3.63Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

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