3jcc

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3jcc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JCC FirstGlance]. <br>
<table><tr><td colspan='2'>[[3jcc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JCC FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3jcb|3jcb]]</div></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jcc OCA], [https://pdbe.org/3jcc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jcc RCSB], [https://www.ebi.ac.uk/pdbsum/3jcc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jcc ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jcc OCA], [https://pdbe.org/3jcc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jcc RCSB], [https://www.ebi.ac.uk/pdbsum/3jcc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jcc ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/CD4_HUMAN CD4_HUMAN]] Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
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[https://www.uniprot.org/uniprot/A0A3B6UDT5_SIV A0A3B6UDT5_SIV]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The human immunodeficiency virus type 1 (HIV-1)/simian immunodeficiency virus (SIV) envelope spike (Env) mediates viral entry into host cells. The V3 loop of the gp120 component of the Env trimer contributes to the coreceptor binding site and is a target for neutralizing antibodies. We used cryo-electron tomography to visualize the binding of CD4 and the V3 loop monoclonal antibody (MAb) 36D5 to gp120 of the SIV Env trimer. Our results show that 36D5 binds gp120 at the base of the V3 loop and suggest that the antibody exerts its neutralization effect by blocking the coreceptor binding site. The antibody does this without altering the dynamics of the spike motion between closed and open states when CD4 is bound. The interaction between 36D5 and SIV gp120 is similar to the interaction between some broadly neutralizing anti-V3 loop antibodies and HIV-1 gp120. Two conformations of gp120 bound with CD4 are revealed, suggesting an intrinsic dynamic nature of the liganded Env trimer. CD4 binding substantially increases the binding of 36D5 to gp120 in the intact Env trimer, consistent with CD4-induced changes in the conformation of gp120 and the antibody binding site. Binding by MAb 36D5 does not substantially alter the proportions of the two CD4-bound conformations. The position of MAb 36D5 at the V3 base changes little between conformations, indicating that the V3 base serves as a pivot point during the transition between these two states.IMPORTANCE Glycoprotein spikes on the surfaces of SIV and HIV are the sole targets available to the immune system for antibody neutralization. Spikes evade the immune system by a combination of a thick layer of polysaccharide on the surface (the glycan shield) and movement between spike domains that masks the epitope conformation. Using SIV virions whose spikes were "decorated" with the primary cellular receptor (CD4) and an antibody (36D5) at part of the coreceptor binding site, we visualized multiple conformations trapped by the rapid freezing step, which were separated using statistical analysis. Our results show that the CD4-induced conformational dynamics of the spike enhances binding of the antibody.
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Structure of Simian Immunodeficiency Virus Envelope Spikes Bound with CD4 and Monoclonal Antibody 36D5.,Hu G, Liu J, Roux KH, Taylor KA J Virol. 2017 Jul 27;91(16). pii: e00134-17. doi: 10.1128/JVI.00134-17. Print, 2017 Aug 15. PMID:28539445<ref>PMID:28539445</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3jcc" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Gp120 3D structures|Gp120 3D structures]]
*[[Gp120 3D structures|Gp120 3D structures]]
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== References ==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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<references/>
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__TOC__
__TOC__
</SX>
</SX>
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[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Simian immunodeficiency virus]]
[[Category: Simian immunodeficiency virus]]
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[[Category: Hu, G]]
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[[Category: Hu G]]
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[[Category: Liu, J]]
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[[Category: Liu J]]
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[[Category: Roux, K]]
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[[Category: Roux K]]
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[[Category: Taylor, K A]]
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[[Category: Taylor KA]]
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[[Category: Aid]]
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[[Category: Cryoelectron tomography]]
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[[Category: Hiv]]
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[[Category: Immunology]]
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[[Category: Viral protein-immune system complex]]
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Revision as of 08:43, 7 February 2024

Structure of Simian Immunodeficiency Virus Envelope Spikes bound with CD4 and Monoclonal Antibody 36D5

3jcc

Proteopedia Page Contributors and Editors (what is this?)

OCA

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