3k55

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<StructureSection load='3k55' size='340' side='right'caption='[[3k55]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
<StructureSection load='3k55' size='340' side='right'caption='[[3k55]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3k55]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_rn4220 Staphylococcus aureus rn4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K55 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3k55]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_RN4220 Staphylococcus aureus subsp. aureus RN4220]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K55 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hlb ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=561307 Staphylococcus aureus RN4220])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Sphingomyelin_phosphodiesterase Sphingomyelin phosphodiesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.12 3.1.4.12] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k55 OCA], [https://pdbe.org/3k55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k55 RCSB], [https://www.ebi.ac.uk/pdbsum/3k55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k55 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k55 OCA], [https://pdbe.org/3k55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k55 RCSB], [https://www.ebi.ac.uk/pdbsum/3k55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k55 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A7LAI8_STAAU A7LAI8_STAAU]
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The 3.35 A resolution crystal structure of a mutant form of the staphylococcal sphingomyelinase beta toxin in which a conserved hydrophobic beta-hairpin has been deleted is reported. It is shown that this mutation induces domain swapping of a C-terminal beta-strand, leading to the formation of dimers linked by a conformationally flexible hinge region. Eight dimers are seen in the asymmetric unit, exhibiting a broad spectrum of conformations trapped in place by intermolecular contacts within the crystal lattice. Furthermore, the 16 monomers within each asymmetric unit exhibit a remarkable heterogeneity in thermal factors, which can be accounted for by the varying degrees to which each monomer interacts with other molecules in the crystal. This structure provides a unique example of the challenges associated with crystallographic study of flexible proteins.
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Structure of a mutant beta toxin from Staphylococcus aureus reveals domain swapping and conformational flexibility.,Kruse AC, Huseby MJ, Shi K, Digre J, Ohlendorf DH, Earhart CA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt, 4):438-41. Epub 2011 Mar 24. PMID:21505235<ref>PMID:21505235</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3k55" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Hemolysin 3D structures|Hemolysin 3D structures]]
*[[Hemolysin 3D structures|Hemolysin 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Sphingomyelin phosphodiesterase]]
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[[Category: Staphylococcus aureus subsp. aureus RN4220]]
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[[Category: Staphylococcus aureus rn4220]]
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[[Category: Digre J]]
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[[Category: Digre, J]]
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[[Category: Earhart CA]]
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[[Category: Earhart, C A]]
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[[Category: Huseby M]]
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[[Category: Huseby, M]]
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[[Category: Kruse AC]]
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[[Category: Kruse, A C]]
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[[Category: Ohlendorf DH]]
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[[Category: Ohlendorf, D H]]
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[[Category: Shi K]]
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[[Category: Shi, K]]
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[[Category: Beta toxin]]
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[[Category: Domain swapping]]
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[[Category: Hemolysin]]
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[[Category: Hydrolase]]
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[[Category: Sphingomyelinase]]
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Revision as of 08:44, 7 February 2024

Structure of beta hairpin deletion mutant of beta toxin from Staphylococcus aureus

PDB ID 3k55

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