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| | <StructureSection load='3m9g' size='340' side='right'caption='[[3m9g]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='3m9g' size='340' side='right'caption='[[3m9g]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3m9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staan Staan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M9G FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3m9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_N315 Staphylococcus aureus subsp. aureus N315]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M9G FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SA1063 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158879 STAAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m9g OCA], [https://pdbe.org/3m9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m9g RCSB], [https://www.ebi.ac.uk/pdbsum/3m9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m9g ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m9g OCA], [https://pdbe.org/3m9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m9g RCSB], [https://www.ebi.ac.uk/pdbsum/3m9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m9g ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A0H3JME9_STAAN A0A0H3JME9_STAAN] |
| - | PASTA (penicillin-binding protein and serine/threonine kinase associated) modules are found in penicillin-binding proteins and bacterial serine/threonine kinases mainly from Gram-positive Firmicutes and Actinobacteria. They may act as extracellular sensors by binding peptidoglycan fragments. We report here the first crystal structure of a multiple-PASTA domain from Ser/Thr kinase, that of the protein serine/threonine kinase 1 (Stk1) from the Firmicute Staphylococcus aureus. The extended conformation of the three PASTA subunits differs strongly from the compact conformation observed in the two-PASTA domain of penicillin-binding protein PBP2x, whereas linear conformations were also reported for two-subunit fragments of the four-PASTA domain of the Actinobacteria Mycobacterium tuberculosis studied by liquid NMR. Thus, a stretched organization appears to be the signature of modular PASTA domains in Ser/Thr kinases. Signal transduction to the kinase domain is supposed to occur via dimerization and ligand binding. A conserved X-shaped crystallographic dimer stabilized by intermolecular interactions between the second PASTA subunits of each monomer is observed in the two crystal forms of Stk1 that we managed to crystallize. Extracellular PASTA domains are composed of at least two subunits, and this molecular assembly is a plausible candidate for the biological dimer. We have also performed docking experiments, which predict that the hinge regions of the PASTA domain can accommodate peptidoglycan. Finally, a three-dimensional homology molecular model of full-length Stk1 was generated, suggesting an interaction between the kinase domain and the cytoplasmic face of the plasma membrane via a eukaryotic-like juxtamembrane domain. A comprehensive activation mechanism for bacterial Ser/Thr kinases is proposed with the support of these structural data.
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| - | | + | |
| - | The Extended Conformation of the 2.9-A Crystal Structure of the Three-PASTA Domain of a Ser/Thr Kinase from the Human Pathogen Staphylococcus aureus.,Paracuellos P, Ballandras A, Robert X, Kahn R, Herve M, Mengin-Lecreulx D, Cozzone AJ, Duclos B, Gouet P J Mol Biol. 2010 Oct 19. PMID:20965199<ref>PMID:20965199</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3m9g" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Non-specific serine/threonine protein kinase]] | + | [[Category: Staphylococcus aureus subsp. aureus N315]] |
| - | [[Category: Staan]]
| + | [[Category: Ballandras A]] |
| - | [[Category: Ballandras, A]] | + | [[Category: Cozzone AJ]] |
| - | [[Category: Cozzone, A J]] | + | [[Category: Creze C]] |
| - | [[Category: Creze, C]] | + | [[Category: Duclos B]] |
| - | [[Category: Duclos, B]] | + | [[Category: Gouet P]] |
| - | [[Category: Gouet, P]] | + | [[Category: Paracuellos P]] |
| - | [[Category: Paracuellos, P]] | + | [[Category: Robert X]] |
| - | [[Category: Robert, X]] | + | |
| - | [[Category: Atp-binding]]
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| - | [[Category: Extracellular domain]]
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| - | [[Category: Kinase]]
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| - | [[Category: Nucleotide-binding]]
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| - | [[Category: Pasta domain]]
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| - | [[Category: Ser/thr kinase]]
| + | |
| - | [[Category: Serine/threonine-protein kinase]]
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| - | [[Category: Staphylococcus aureus]]
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| - | [[Category: Stk1]]
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| - | [[Category: Transferase]]
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