1s32
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(New page: 200px<br /> <applet load="1s32" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s32, resolution 2.05Å" /> '''Molecular Recogniti...)
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Revision as of 17:03, 12 November 2007
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Molecular Recognition of the Nucleosomal 'Supergroove'
Overview
Chromatin is the physiological substrate in all processes involving, eukaryotic DNA. By organizing 147 base pairs of DNA into two tight, superhelical coils, the nucleosome generates an architecture where DNA, regions that are 80 base pairs apart on linear DNA are brought into close, proximity, resulting in the formation of DNA "supergrooves." Here, we, report the design of a hairpin polyamide dimer that targets one such, supergroove. The 2-A crystal structure of the nucleosome-polyamide complex, shows that the bivalent "clamp" effectively crosslinks the two gyres of, the DNA superhelix, improves positioning of the DNA on the histone, octamer, and stabilizes the nucleosome against dissociation. Our findings, identify nucleosomal supergrooves as platforms for molecular recognition, of condensed eukaryotic DNA. In vivo, supergrooves may foster synergistic, protein-protein interactions by bringing two regulatory elements into, juxtaposition. Because supergroove formation is independent of the, translational position of the DNA on the histone octamer, accurate, nucleosome positioning over regulatory elements is not required for, supergroove participation in eukaryotic gene regulation.
About this Structure
1S32 is a Protein complex structure of sequences from Xenopus laevis with MN, CL, IMT and OGG as ligands. Full crystallographic information is available from OCA.
Reference
Molecular recognition of the nucleosomal "supergroove"., Edayathumangalam RS, Weyermann P, Gottesfeld JM, Dervan PB, Luger K, Proc Natl Acad Sci U S A. 2004 May 4;101(18):6864-9. Epub 2004 Apr 20. PMID:15100411
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