1kso

<table><tr><td colspan='2'>[[1kso]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KSO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KSO FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kso OCA], [https://pdbe.org/1kso PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kso RCSB], [https://www.ebi.ac.uk/pdbsum/1kso PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kso ProSAT]</span></td></tr>

[[https://www.uniprot.org/uniprot/S10A3_HUMAN S10A3_HUMAN]] Binds both calcium and zinc. May be involved in calcium-dependent cuticle cell differentiation, hair shaft and hair cuticular barrier formation.<ref>PMID:18083705</ref>

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

S100 proteins are involved in metal-dependent intracellular signalling. Metal-free S100A3, a cysteine-rich Ca(2+)- and Zn(2+)-binding protein, has been crystallized by vapour diffusion under the strict exclusion of oxygen and in the absence of divalent metal ions. Metal binding induces large conformational changes, rendering the apo-S100A3 crystals very sensitive to various metal compounds. Therefore, the structure was solved by MIRAS phasing using potassium iodide and xenon derivatives. Iodide replaces a water molecule at the surface of the S100A3 protein, whereas xenon binds in a hydrophobic cavity at the dimer interface. Despite significant non-isomorphism, the combination of both derivatives was sufficient for structure determination. The overall apo-S100A3 structure resembles the structures of metal-free S100B and S100A6 solution structures. In contrast to the NMR structures, the EF-hand loops are well ordered in the apo-S100A3 crystal structure. In the N-terminal pseudo-EF-hand loop a water molecule occupies the position of the Ca(2+) ion. The C-terminal canonical EF-hand loop shows an extended conformation and a different helix arrangement to other S100/metal complex crystal structures.

Metal-free MIRAS phasing: structure of apo-S100A3.,Mittl PR, Fritz G, Sargent DF, Richmond TJ, Heizmann CW, Grutter MG Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1255-61. Epub 2002, Jul 20. PMID:12136135<ref>PMID:12136135</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

<div class="pdbe-citations 1kso" style="background-color:#fffaf0;"></div>

[[Category: Human]]

[[Category: Fritz, G]]

[[Category: Grutter, M G]]

[[Category: Heizmann, C W]]

[[Category: Mittl, P R]]

[[Category: Richmond, T J]]

[[Category: Sargent, D F]]

[[Category: Zn2+ binding protein]]