1s8c

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Revision as of 17:04, 12 November 2007

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spinqualitylabelsall models 1s8c, resolution 2.19Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of human heme oxygenase in a complex with biliverdine

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Heme oxygenase oxidatively cleaves heme to biliverdin, leading to the, release of iron and CO through a process in which the heme participates, both as a cofactor and as a substrate. Here we report the crystal, structure of the product, iron-free biliverdin, in a complex with human, HO-1 at 2.19 A. Structural comparisons of the human biliverdin-HO-1, structure with its heme complex and the recently published rat HO-1, structure in a complex with the biliverdin-iron chelate [Sugishima, M., Sakamoto, H., Higashimoto, Y., Noguchi, M., and Fukuyama, K. (2003) J., Biol. Chem. 278, 32352-32358] show two major differences. First, in the, absence of an Fe-His bond and solvent structure in the active site, the, distal and proximal helices relax and adopt an "open" conformation which, most likely encourages biliverdin release. Second, iron-free biliverdin, occupies a different position and orientation relative to heme and the, biliverdin-iron complex. Biliverdin adopts a more linear conformation and, moves from the heme site to an internal cavity. These structural results, provide insight into the rate-limiting step in HO-1 catalysis, which is, product, biliverdin, release.

Disease

Known diseases associated with this structure: Epiphyseal dysplasia, multiple, 5 OMIM:[602109], Heme oxygenase-1 deficiency OMIM:[141250], Osteoarthritis, hand, susceptibility to OMIM:[602109], Spondyloepimetaphyseal dysplasia OMIM:[602109]

About this Structure

1S8C is a Single protein structure of sequence from Homo sapiens with BLA as ligand. Active as Heme oxygenase, with EC number 1.14.99.3 Full crystallographic information is available from OCA.

Reference

Crystal structure of human heme oxygenase-1 in a complex with biliverdin., Lad L, Friedman J, Li H, Bhaskar B, Ortiz de Montellano PR, Poulos TL, Biochemistry. 2004 Apr 6;43(13):3793-801. PMID:15049686

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