1q7l
From Proteopedia
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'''Zn-binding domain of the T347G mutant of human aminoacylase-I''' | '''Zn-binding domain of the T347G mutant of human aminoacylase-I''' | ||
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[[Category: Lunin, V V.]] | [[Category: Lunin, V V.]] | ||
[[Category: Menard, R.]] | [[Category: Menard, R.]] | ||
| - | [[Category: | + | [[Category: Aminoacylase-1]] |
| - | [[Category: | + | [[Category: Catalysis]] |
| - | [[Category: | + | [[Category: Enzyme dimerization]] |
| - | [[Category: | + | [[Category: Site-directed mutagenesis]] |
| - | [[Category: | + | [[Category: Structure comparison]] |
| - | [[Category: | + | [[Category: Zinc]] |
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Revision as of 02:58, 3 May 2008
Zn-binding domain of the T347G mutant of human aminoacylase-I
Contents |
Overview
Members of the aminoacylase-1 (Acy1)/M20 family of aminoacylases and exopeptidases exist as either monomers or homodimers. They contain a zinc-binding domain and a second domain mediating dimerization in the latter case. The roles that both domains play in catalysis have been investigated for human Acy1 (hAcy1) by x-ray crystallography and by site-directed mutagenesis. Structure comparison of the dinuclear zinc center in a mutant of hAcy1 reported here with dizinc centers in related enzymes points to a difference in zinc ligation in the Acy1/M20 family. Mutational analysis supports catalytic roles of zinc ions, a vicinal glutamate, and a histidine from the dimerization domain. By complementing different active site mutants of hAcy1, we show that catalysis occurs at the dimer interface. Reinterpretation of the structure of a monomeric homolog, peptidase V, reveals that a domain insertion mimics dimerization. We conclude that monomeric and dimeric Acy1/M20 family members share a unique active site architecture involving both enzyme domains. The study may provide means to improve homologous carboxypeptidase G2 toward application in antibody-directed enzyme prodrug therapy.
Disease
Known disease associated with this structure: Aminoacylase 1 deficiency OMIM:[104620]
About this Structure
1Q7L is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Essential roles of zinc ligation and enzyme dimerization for catalysis in the aminoacylase-1/M20 family., Lindner HA, Lunin VV, Alary A, Hecker R, Cygler M, Menard R, J Biol Chem. 2003 Nov 7;278(45):44496-504. Epub 2003 Aug 21. PMID:12933810 Page seeded by OCA on Sat May 3 05:58:09 2008
