1sty
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
<StructureSection load='1sty' size='340' side='right'caption='[[1sty]], [[Resolution|resolution]] 1.67Å' scene=''> | <StructureSection load='1sty' size='340' side='right'caption='[[1sty]], [[Resolution|resolution]] 1.67Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1sty]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1sty]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1STY FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=THP:THYMIDINE-3,5-DIPHOSPHATE'>THP</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sty OCA], [https://pdbe.org/1sty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sty RCSB], [https://www.ebi.ac.uk/pdbsum/1sty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sty ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sty OCA], [https://pdbe.org/1sty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sty RCSB], [https://www.ebi.ac.uk/pdbsum/1sty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sty ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/NUC_STAAU NUC_STAAU] Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 20: | Line 20: | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sty ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sty ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The x-ray crystal structure of a mutant of staphylococcal nuclease that contains a single glycine residue inserted in the C-terminal alpha-helix has been solved to 1.67 A resolution and refined to a crystallographic R value of 0.170. This inserted glycine residue is accommodated in the alpha-helix by formation of a previously uncharacterized bulge, which we term the alpha aneurism. A conformational search of known protein structures has identified the alpha aneurism in a number of protein families, including the histocompatibility antigens and hemoglobins. | ||
| - | |||
| - | The alpha aneurism: a structural motif revealed in an insertion mutant of staphylococcal nuclease.,Keefe LJ, Sondek J, Shortle D, Lattman EE Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3275-9. PMID:8475069<ref>PMID:8475069</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1sty" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Staphylococcal nuclease 3D structures|Staphylococcal nuclease 3D structures]] | *[[Staphylococcal nuclease 3D structures|Staphylococcal nuclease 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Staphylococcus aureus]] |
| - | [[Category: Keefe | + | [[Category: Keefe LJ]] |
| - | [[Category: Lattman | + | [[Category: Lattman EE]] |
Current revision
THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE
| |||||||||||
