1q97
From Proteopedia
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'''The structure of the Saccharomyces cerevisiae SR protein kinase, Sky1p, with bound ATP''' | '''The structure of the Saccharomyces cerevisiae SR protein kinase, Sky1p, with bound ATP''' | ||
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[[Category: Nolen, B.]] | [[Category: Nolen, B.]] | ||
[[Category: Vu, D.]] | [[Category: Vu, D.]] | ||
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Revision as of 03:01, 3 May 2008
The structure of the Saccharomyces cerevisiae SR protein kinase, Sky1p, with bound ATP
Overview
Conformational changes are thought to play a key role in the function of active protein kinases, although little is known about how these changes relate to the mechanism of phosphorylation. Here we present four high-resolution structures of a single crystal form of Sky1p, a constitutively active serine kinase implicated in yeast RNA processing, each in a different state of nucleotide binding. By comparing the apoenzyme structure to the ADP- and ATP-bound Sky1p structures, we have revealed conformational changes caused by ATP binding or conversion from nucleotide reactant to product. Rotation of the small lobe of the kinase closes the cleft upon binding, allowing the nucleotide to interact with residues from both lobes of the kinase, although some interactions thought to be important for phosphotransfer are missing in the ATP-containing structure. In the apoenzyme, a kinase-conserved phosphate-anchoring loop is in a twisted conformation that is incompatible with ADP and ATP binding, providing a potential mechanism for facilitating ADP release in Sky1p. The nonhydrolyzable ATP analogue AMP-PNP binds in a unique mode that fails to induce lobe closure. This observation, along with comparisons between the two independent molecules in the asymmetric unit of each structure, has provided new molecular details about how the nucleotide binds and induces closure. Finally, we have used mutational analysis to establish the importance of a glycine within the linker that connects the two lobes of Sky1p.
About this Structure
1Q97 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Nucleotide-induced conformational changes in the Saccharomyces cerevisiae SR protein kinase, Sky1p, revealed by X-ray crystallography., Nolen B, Ngo J, Chakrabarti S, Vu D, Adams JA, Ghosh G, Biochemistry. 2003 Aug 19;42(32):9575-85. PMID:12911299 Page seeded by OCA on Sat May 3 06:01:33 2008
