1q9g

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[[Image:1q9g.jpg|left|200px]]
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{{Structure
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|PDB= 1q9g |SIZE=350|CAPTION= <scene name='initialview01'>1q9g</scene>
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The line below this paragraph, containing "STRUCTURE_1q9g", creates the "Structure Box" on the page.
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|GENE= OMPX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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{{STRUCTURE_1q9g| PDB=1q9g | SCENE= }}
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|RELATEDENTRY=[[1orm|1ORM]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q9g OCA], [http://www.ebi.ac.uk/pdbsum/1q9g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1q9g RCSB]</span>
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'''NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES'''
'''NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES'''
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[[Category: Wider, G.]]
[[Category: Wider, G.]]
[[Category: Wuthrich, K.]]
[[Category: Wuthrich, K.]]
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[[Category: detergent]]
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[[Category: Detergent]]
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[[Category: dhpc]]
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[[Category: Dhpc]]
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[[Category: lipid]]
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[[Category: Lipid]]
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[[Category: membrane protein]]
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[[Category: Membrane protein]]
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[[Category: micelle]]
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[[Category: Micelle]]
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[[Category: nmr]]
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[[Category: Nmr]]
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[[Category: ompx]]
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[[Category: Ompx]]
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[[Category: trosy]]
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[[Category: Trosy]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:02:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:10:42 2008''
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Revision as of 03:02, 3 May 2008

Image:1q9g.jpg

Template:STRUCTURE 1q9g

NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES


Overview

The structure of the integral membrane protein OmpX from Escherichia coli reconstituted in 60 kDa DHPC micelles (OmpX/DHPC) was calculated from 526 NOE upper limit distance constraints. The structure determination was based on complete sequence-specific assignments for the amide protons and the Val, Leu, and Ile(delta1) methyl groups in OmpX, which were selectively protonated on a perdeuterated background. The solution structure of OmpX in the DHPC micelles consists of a well-defined, eight-stranded antiparallel beta-barrel, with successive pairs of beta-strands connected by mobile loops. Several long-range NOEs observed outside of the transmembrane barrel characterize an extension of a four-stranded beta-sheet beyond the height of the barrel. This protruding beta-sheet is believed to be involved in intermolecular interactions responsible for the biological functions of OmpX. The present approach for de novo structure determination should be quite widely applicable to membrane proteins reconstituted in mixed micelles with overall molecular masses up to about 100 kDa, and may also provide a platform for additional functional studies.

About this Structure

1Q9G is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

NMR structure of the integral membrane protein OmpX., Fernandez C, Hilty C, Wider G, Guntert P, Wuthrich K, J Mol Biol. 2004 Mar 5;336(5):1211-21. PMID:15037080 Page seeded by OCA on Sat May 3 06:02:03 2008

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