1qaz

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[[Image:1qaz.gif|left|200px]]
[[Image:1qaz.gif|left|200px]]
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{{Structure
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|PDB= 1qaz |SIZE=350|CAPTION= <scene name='initialview01'>1qaz</scene>, resolution 1.78&Aring;
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The line below this paragraph, containing "STRUCTURE_1qaz", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Urea_carboxylase Urea carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.6 6.3.4.6] </span>
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{{STRUCTURE_1qaz| PDB=1qaz | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qaz OCA], [http://www.ebi.ac.uk/pdbsum/1qaz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qaz RCSB]</span>
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'''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION'''
'''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION'''
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[[Category: Urea carboxylase]]
[[Category: Urea carboxylase]]
[[Category: Yoon, H J.]]
[[Category: Yoon, H J.]]
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[[Category: alginate lyase]]
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[[Category: Alginate lyase]]
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[[Category: crystal]]
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[[Category: Crystal]]
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[[Category: x-ray structure]]
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[[Category: X-ray structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:05:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:11:16 2008''
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Revision as of 03:05, 3 May 2008

Image:1qaz.gif

Template:STRUCTURE 1qaz

CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION


Overview

The three-dimensional structure of alginate lyase A1-III (ALYIII) from a Sphingomonas species A1 was determined by X-ray crystallography. The enzyme was crystallized by the hanging-drop vapour-diffusion method in the presence of 49% ammonium sulfate at 20 degrees C. The crystals are monoclinic and belong to the space group C2 with unit cell dimensions of a=49.18 A, b=93.08 A, c=82.10 A and beta=104.12 degrees. There was one molecule of alginate lyase in the asymmetric unit of the crystal. The diffraction data up to 1. 71 A were collected with Rsymof 5.0%. The crystal structure of ALYIII was solved by the multiple isomorphous replacement method and refined at 1.78 A resolution using X-PLOR with a final R -factor of 18.0% for 10.0 to 1.78 A resolution data. The refined model of ALYIII contained 351 amino acid residues, 299 water molecules and two sulfate ions. The three-dimensional structure of ALYIII was abundant in helices and had a deep tunnel-like cleft in a novel (alpha6/alpha5)-barrel structure, which was similar to the (alpha6/alpha6)-barrel found in glucoamylase and cellulase. This structure presented the possibility that alginate molecules might penetrate into the cleft to interact with the catalytic site of ALYIII.

About this Structure

1QAZ is a Single protein structure of sequence from Sphingomonas sp.. Full crystallographic information is available from OCA.

Reference

Crystal structure of alginate lyase A1-III from Sphingomonas species A1 at 1.78 A resolution., Yoon HJ, Mikami B, Hashimoto W, Murata K, J Mol Biol. 1999 Jul 9;290(2):505-14. PMID:10390348 Page seeded by OCA on Sat May 3 06:05:10 2008

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