2cbf
From Proteopedia
(Difference between revisions)
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<StructureSection load='2cbf' size='340' side='right'caption='[[2cbf]], [[Resolution|resolution]] 3.10Å' scene=''> | <StructureSection load='2cbf' size='340' side='right'caption='[[2cbf]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[2cbf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CBF FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr> |
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [https://pdbe.org/2cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [https://www.ebi.ac.uk/pdbsum/2cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cbf ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cbf OCA], [https://pdbe.org/2cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cbf RCSB], [https://www.ebi.ac.uk/pdbsum/2cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cbf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/CBIF_PRIMG CBIF_PRIMG] Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.<ref>PMID:23922391</ref> | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cbf ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cbf ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Biosynthesis of the corrin ring of vitamin B12 requires the action of six S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been determined to a resolution of 2.4 A. CbiF contains two alphabeta domains forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The location of AdoHcy and a number of conserved residues, helps define the precorrin binding site. A second crystal form determined at 3.1 A resolution highlights the flexibility of two loops around this site. CbiF employs a unique mode of AdoHcy binding and represents a new class of transmethylase. | ||
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| - | The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase.,Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:9665173<ref>PMID:9665173</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2cbf" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Atcc 14581]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Priestia megaterium]] |
| - | [[Category: | + | [[Category: Raux E]] |
| - | [[Category: | + | [[Category: Schubert HL]] |
| - | [[Category: | + | [[Category: Warren MJ]] |
| - | [[Category: | + | [[Category: Wilson KS]] |
| - | [[Category: | + | [[Category: Woodcock SC]] |
| - | + | ||
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Current revision
THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF, FROM BACILLUS MEGATERIUM, WITH THE HIS-TAG CLEAVED OFF
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