2gic
From Proteopedia
(Difference between revisions)
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<StructureSection load='2gic' size='340' side='right'caption='[[2gic]], [[Resolution|resolution]] 2.92Å' scene=''> | <StructureSection load='2gic' size='340' side='right'caption='[[2gic]], [[Resolution|resolution]] 2.92Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2gic]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[2gic]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus Vesicular stomatitis Indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GIC FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.92Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gic OCA], [https://pdbe.org/2gic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gic RCSB], [https://www.ebi.ac.uk/pdbsum/2gic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gic ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gic OCA], [https://pdbe.org/2gic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gic RCSB], [https://www.ebi.ac.uk/pdbsum/2gic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gic ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/NCAP_VSIVA NCAP_VSIVA] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity). | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gic ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gic ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Vesicular stomatitis virus is a negative-stranded RNA virus. Its nucleoprotein (N) binds the viral genomic RNA and is involved in multiple functions including transcription, replication, and assembly. We have determined a 2.9 angstrom structure of a complex containing 10 molecules of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a cavity at the interface between two lobes of the N protein. This serves to protect the RNA in the absence of polynucleotide synthesis. For the RNA to be accessed, some conformational change in the N protein should be necessary. | ||
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| - | Structure of the vesicular stomatitis virus nucleoprotein-RNA complex.,Green TJ, Zhang X, Wertz GW, Luo M Science. 2006 Jul 21;313(5785):357-60. Epub 2006 Jun 15. PMID:16778022<ref>PMID:16778022</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2gic" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Vesicular stomatitis Indiana virus]] |
| - | [[Category: Green | + | [[Category: Green TJ]] |
| - | [[Category: Luo | + | [[Category: Luo M]] |
| - | [[Category: Wertz | + | [[Category: Wertz GW]] |
| - | [[Category: Zhang | + | [[Category: Zhang X]] |
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Current revision
Crystal Structure of a vesicular stomatitis virus nucleocapsid-RNA complex
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