1qbp
From Proteopedia
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[[Image:1qbp.gif|left|200px]] | [[Image:1qbp.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_1qbp| PDB=1qbp | SCENE= }} | |
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'''CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS''' | '''CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QBP OCA]. | |
==Reference== | ==Reference== | ||
Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability., Anderson AC, O'Neil RH, Filman DJ, Frederick CA, Biochemistry. 1999 Sep 28;38(39):12577-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504226 10504226] | Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability., Anderson AC, O'Neil RH, Filman DJ, Frederick CA, Biochemistry. 1999 Sep 28;38(39):12577-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10504226 10504226] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Anderson, A C.]] | [[Category: Anderson, A C.]] | ||
[[Category: Filman, D J.]] | [[Category: Filman, D J.]] | ||
[[Category: Frederick, C A.]] | [[Category: Frederick, C A.]] | ||
[[Category: Neil, R H.O.]] | [[Category: Neil, R H.O.]] | ||
| - | [[Category: | + | [[Category: Brominated cytosine]] |
| - | [[Category: | + | [[Category: Mismatched base pair]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:06:21 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:06, 3 May 2008
CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS
Overview
The X-ray crystal structure of a brominated RNA helix with four mismatched base pairs and sequence r(UG(Br)C(Br)CAGUUCGCUGGC)(2) was determined to 2.1 A using the methods of multiwavelength anomalous diffraction (MAD) applied to the bromine K-absorption edge. There are three molecules in the asymmetric unit with unique crystal-packing environments, revealing true conformational variability at high resolution for this sequence. The structure shows that the sequence itself does not define a consistent pattern of solvent molecules, with the exception of the mismatched base pairs, implying that specific RNA-protein interactions would occur only with the nucleotides. There are a number of significant tertiary interactions, some of which are a result of the brominated base pairs and others that are directly mediated by the RNA 2' hydroxyl groups. The mismatched base pairs exhibit a solvent network as well as a stacking pattern with their nearest neighbors that validate previous thermodynamic analysis.
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability., Anderson AC, O'Neil RH, Filman DJ, Frederick CA, Biochemistry. 1999 Sep 28;38(39):12577-85. PMID:10504226 Page seeded by OCA on Sat May 3 06:06:21 2008
