3nv0

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<StructureSection load='3nv0' size='340' side='right'caption='[[3nv0]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
<StructureSection load='3nv0' size='340' side='right'caption='[[3nv0]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3nv0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NV0 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3nv0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NV0 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C15H11.6, nxf-2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL]), nxt-1, nxt1, Y71F9AM.5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nv0 OCA], [https://pdbe.org/3nv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nv0 RCSB], [https://www.ebi.ac.uk/pdbsum/3nv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nv0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nv0 OCA], [https://pdbe.org/3nv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nv0 RCSB], [https://www.ebi.ac.uk/pdbsum/3nv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nv0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/NXF2_CAEEL NXF2_CAEEL]] May be involved in the export of cellular mRNA to the cytoplasm (By similarity). [[https://www.uniprot.org/uniprot/NXT1_CAEEL NXT1_CAEEL]] Stimulator of protein export for NES-containing proteins. Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA (By similarity).
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[https://www.uniprot.org/uniprot/NXF2_CAEEL NXF2_CAEEL] May be involved in the export of cellular mRNA to the cytoplasm (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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NXF1-like members of the NXF (nuclear export factor) family orchestrate bulk nuclear export of mRNA, while functionally distinct NXF variant proteins carry out separate substrate-specific and tissue-specific RNA regulation. Metazoan organisms possess at least one NXF1-like gene and one or more NXF variant genes. Heterodimerization of both proteins with the NXT (NTF2-related export) protein is central to NXF family function; however, given the multiplicity of NXF/NXT complexes, the specificity and mechanism of heterodimerization remain unclear. Here, we report the structural and functional analyses of the Caenorhabditis elegans NXF variant ceNXF2 bound to ceNXT1. Contacts crucial for NXF/NXT heterodimer stability and specificity, including a probable site for phosphoregulation, have been identified. The ceNXF2 NTF2 domain bears at least two nucleoporin (Nup) binding pockets necessary for the colocalization of ceNXF2/ceNXT1 at the nuclear envelope. Unexpectedly, one Nup binding pocket is formed at the heterodimer interface of the ceNXF2/ceNXT1 complex, demonstrating that NXT binding directly regulates NXF function.
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The structure of the NXF2/NXT1 heterodimeric complex reveals the combined specificity and versatility of the NTF2-like fold.,Kerkow DE, Carmel AB, Menichelli E, Ambrus G, Hills RD Jr, Gerace L, Williamson JR J Mol Biol. 2012 Jan 27;415(4):649-65. doi: 10.1016/j.jmb.2011.11.027. Epub 2011 , Nov 22. PMID:22123199<ref>PMID:22123199</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3nv0" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Caeel]]
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[[Category: Caenorhabditis elegans]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Carmel, A B]]
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[[Category: Carmel AB]]
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[[Category: Kerkow, D E]]
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[[Category: Kerkow DE]]
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[[Category: Williamson, J R]]
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[[Category: Williamson JR]]
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[[Category: Beta sheet heterodimer interface]]
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[[Category: Cation-pi interaction]]
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[[Category: Intramonomer buried water]]
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[[Category: Mrna transport]]
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[[Category: Novel surface exposed pocket]]
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[[Category: Ntf2-like domain]]
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[[Category: Ntf2-plug]]
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[[Category: Nucleoporin binding pocket]]
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[[Category: Nucleus]]
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[[Category: Nxf-loop]]
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[[Category: Pi-pi interaction]]
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[[Category: Rna binding-protein transport complex]]
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[[Category: Rna-binding]]
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[[Category: Water mediated interface]]
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Current revision

Crystal structure and mutational analysis of the NXF2/NXT1 heterodimeric complex from caenorhabditis elegans at 1.84 A resolution

PDB ID 3nv0

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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