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3o00

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Current revision (10:02, 14 February 2024) (edit) (undo)
 
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<StructureSection load='3o00' size='340' side='right'caption='[[3o00]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
<StructureSection load='3o00' size='340' side='right'caption='[[3o00]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3o00]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O00 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3o00]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O00 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3nzz|3nzz]], [[3o01|3o01]], [[3o02|3o02]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sipD, sspD, STM2883 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o00 OCA], [https://pdbe.org/3o00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o00 RCSB], [https://www.ebi.ac.uk/pdbsum/3o00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o00 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o00 OCA], [https://pdbe.org/3o00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o00 RCSB], [https://www.ebi.ac.uk/pdbsum/3o00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o00 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/SIPD_SALTY SIPD_SALTY]] Required for translocation of effector proteins via the type III secretion system SPI-1, which is essential for an efficient bacterial internalization. Probably acts by modulating the secretion of SipA, SipB, and SipC.<ref>PMID:8522512</ref> <ref>PMID:9194702</ref>
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[https://www.uniprot.org/uniprot/SIPD_SALTY SIPD_SALTY] Required for translocation of effector proteins via the type III secretion system SPI-1, which is essential for an efficient bacterial internalization. Probably acts by modulating the secretion of SipA, SipB, and SipC.<ref>PMID:8522512</ref> <ref>PMID:9194702</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The type III secretion system (T3SS) is a protein injection nanomachinery required for virulence by many human pathogenic bacteria including Salmonella and Shigella. An essential component of the T3SS is the tip protein and the Salmonella SipD and the Shigella IpaD tip proteins interact with bile salts, which serve as environmental sensors for these enteric pathogens. SipD and IpaD have long central coiled coils and their N-terminal regions form alpha-helical hairpins and a short helix alpha3 that pack against the coiled coil. Using AutoDock, others have predicted that the bile salt deoxycholate binds IpaD in a cleft formed by the alpha-helical hairpin and its long central coiled coil. NMR chemical shift mapping, however, indicated that the SipD residues most affected by bile salts are located in a disordered region near helix alpha3. Thus, how bile salts interact with SipD and IpaD is unclear. Here, we report the crystal structures of SipD in complex with the bile salts deoxycholate and chenodeoxycholate. Bile salts bind SipD in a region different from what was predicted for IpaD. In SipD, bile salts bind part of helix alpha3 and the C-terminus of the long central coiled coil, towards the C-terminus of the protein. We discuss the biological implication of the differences in how bile salts interact with SipD and IpaD.
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The crystal structures of the Salmonella type III secretion system tip protein SipD in complex with deoxycholate and chenodeoxycholate.,Chatterjee S, Zhong D, Nordhues BA, Battaile KP, Lovell SW, De Guzman RN Protein Sci. 2010 Oct 28. PMID:21031487<ref>PMID:21031487</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3o00" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus typhimurium loeffler 1892]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Battaile, K P]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Chatterjee, S]]
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[[Category: Battaile KP]]
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[[Category: DeGuzman, R N]]
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[[Category: Chatterjee S]]
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[[Category: Lovell, S]]
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[[Category: DeGuzman RN]]
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[[Category: Nordhues, B A]]
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[[Category: Lovell S]]
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[[Category: Zhong, D]]
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[[Category: Nordhues BA]]
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[[Category: Bacterial needle tip protein]]
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[[Category: Zhong D]]
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[[Category: Cell invasion]]
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[[Category: Coiled-coil]]
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[[Category: Prgi]]
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[[Category: Sipb]]
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[[Category: Sipc]]
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Current revision

Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD-C244S

PDB ID 3o00

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