3ob8

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<StructureSection load='3ob8' size='340' side='right'caption='[[3ob8]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='3ob8' size='340' side='right'caption='[[3ob8]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ob8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OB8 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ob8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OB8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OB8 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3oba|3oba]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLLA0B14883g, LAC4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28985 ATCC 56498])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ob8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ob8 OCA], [https://pdbe.org/3ob8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ob8 RCSB], [https://www.ebi.ac.uk/pdbsum/3ob8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ob8 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ob8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ob8 OCA], [https://pdbe.org/3ob8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ob8 RCSB], [https://www.ebi.ac.uk/pdbsum/3ob8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ob8 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/BGAL_KLULA BGAL_KLULA]
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beta-Galactosidase or lactase is a very important enzyme in the food industry, being that from the yeast Kluyveromyces lactis the most widely used. Here we report its three-dimensional structure both in the free state and complexed with the product galactose. The monomer folds into five domains in a pattern conserved with the prokaryote enzymes of the GH2 family, although two long insertions in domains 2 and 3 are unique and related to oligomerization and specificity. The tetrameric enzyme is a dimer of dimers, with higher dissociation energy for the dimers than for its assembly. Two active centers are located at the interface within each dimer in a narrow channel. The insertion at domain 3 protrudes into this channel and makes putative links with the aglycone moiety of docked lactose. In spite of common structural features related to function, the determinants of the reaction mechanism proposed for Escherichia coli beta-galactosidase are not found in the active site of the K. lactis enzyme. This is the first X-ray crystal structure for a beta-galactosidase used in food processing.
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Structural basis of specificity in tetrameric Kluyveromyces lactis beta-galactosidase.,Pereira-Rodriguez A, Fernandez-Leiro R, Gonzalez-Siso MI, Cerdan ME, Becerra M, Sanz-Aparicio J J Struct Biol. 2011 Dec 13. PMID:22193516<ref>PMID:22193516</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ob8" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Galactosidase 3D structures|Galactosidase 3D structures]]
*[[Galactosidase 3D structures|Galactosidase 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 56498]]
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[[Category: Kluyveromyces lactis]]
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[[Category: Beta-galactosidase]]
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Becerra, M]]
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[[Category: Becerra M]]
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[[Category: Cerdan, M E]]
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[[Category: Cerdan ME]]
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[[Category: Fernandez-Leiro, R]]
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[[Category: Fernandez-Leiro R]]
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[[Category: Gonzalez-Siso, I]]
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[[Category: Gonzalez-Siso I]]
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[[Category: Pereira-Rodriguez, A]]
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[[Category: Pereira-Rodriguez A]]
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[[Category: Sanz-Aparicio, J]]
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[[Category: Sanz-Aparicio J]]
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[[Category: Galactose]]
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[[Category: Gh2]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Tetramer]]
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[[Category: Tim barrel]]
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Current revision

Structure of the beta-galactosidase from Kluyveromyces lactis in complex with galactose

PDB ID 3ob8

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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