1qet
From Proteopedia
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'''TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES''' | '''TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QET OCA]. | |
==Reference== | ==Reference== | ||
Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2., McDowell JA, He L, Chen X, Turner DH, Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9201950 9201950] | Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2., McDowell JA, He L, Chen X, Turner DH, Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9201950 9201950] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Chen, X.]] | [[Category: Chen, X.]] | ||
[[Category: He, L.]] | [[Category: He, L.]] | ||
[[Category: Mcdowell, J A.]] | [[Category: Mcdowell, J A.]] | ||
[[Category: Turner, D H.]] | [[Category: Turner, D H.]] | ||
| - | [[Category: | + | [[Category: G:u mismatch]] |
| - | [[Category: | + | [[Category: Ribonucleic acid]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:10:54 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:10, 3 May 2008
TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES
Overview
The symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The three-dimensional structures of two representative duplexes, (rGGAGUUCC)2 and (rGGAUGUCC)2, were determined by two-dimensional NMR and a simulated annealing protocol. Local deviations are similar to other intrahelical GU mismatches with little effect on backbone torsion angles and a slight overtwisting between the base pair 5' of the G of the mismatch and the mismatch itself. Comparisons of the resulting stacking patterns along with electrostatic potential maps suggest that interactions between highly negative electrostatic regions between base pairs may play a role in the observed thermodynamic differences.
About this Structure
Full crystallographic information is available from OCA.
Reference
Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2., McDowell JA, He L, Chen X, Turner DH, Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:9201950 Page seeded by OCA on Sat May 3 06:10:54 2008
