2qtk
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
<StructureSection load='2qtk' size='340' side='right'caption='[[2qtk]], [[Resolution|resolution]] 2.80Å' scene=''> | <StructureSection load='2qtk' size='340' side='right'caption='[[2qtk]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2qtk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[2qtk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QTK FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene></td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qtk OCA], [https://pdbe.org/2qtk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qtk RCSB], [https://www.ebi.ac.uk/pdbsum/2qtk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qtk ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qtk OCA], [https://pdbe.org/2qtk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qtk RCSB], [https://www.ebi.ac.uk/pdbsum/2qtk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qtk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9HUR5_PSEAE Q9HUR5_PSEAE] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
| Line 18: | Line 20: | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qtk ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qtk ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | In Gram-negative bacteria that do not have porins, most water-soluble and small molecules are taken up by substrate-specific channels belonging to the OprD family. We report here the X-ray crystal structure of OpdK, an OprD family member implicated in the uptake of vanillate and related small aromatic acids. The OpdK structure reveals a monomeric, 18-stranded beta barrel with a kidney-shaped central pore. The OpdK pore constriction is relatively wide for a substrate-specific channel (approximately 8 A diameter), and it is lined by a positively charged patch of arginine residues on one side and an electronegative pocket on the opposite side-features likely to be important for substrate selection. Single-channel electrical recordings of OpdK show binding of vanillate to the channel, and they suggest that OpdK forms labile trimers in the outer membrane. Comparison of the OpdK structure with that of Pseudomonas aeruginosa OprD provides the first qualitative insights into the different substrate specificities of these closely related channels. | ||
| - | |||
| - | Crystal structure of the outer membrane protein OpdK from Pseudomonas aeruginosa.,Biswas S, Mohammad MM, Movileanu L, van den Berg B Structure. 2008 Jul;16(7):1027-35. PMID:18611376<ref>PMID:18611376</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2qtk" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Porin 3D structures|Porin 3D structures]] | *[[Porin 3D structures|Porin 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Pseudomonas aeruginosa | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | + | [[Category: Biswas S]] | |
| - | [[Category: Biswas | + | [[Category: Van den Berg B]] |
| - | [[Category: | + | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
Current revision
Crystal Structure of the outer membrane protein opdK from Pseudomonas aeruginosa
| |||||||||||
