2quw
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2quw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2quw OCA], [https://pdbe.org/2quw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2quw RCSB], [https://www.ebi.ac.uk/pdbsum/2quw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2quw ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2quw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2quw OCA], [https://pdbe.org/2quw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2quw RCSB], [https://www.ebi.ac.uk/pdbsum/2quw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2quw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | We have obtained precatalytic (enzyme-substrate complex) and postcatalytic (enzyme-product complex) crystal structures of an active full-length hammerhead RNA that cleaves in the crystal. Using the natural satellite tobacco ringspot virus hammerhead RNA sequence, the self-cleavage reaction was modulated by substituting the general base of the ribozyme, G12, with A12, a purine variant with a much lower pKa that does not significantly perturb the ribozyme's atomic structure. The active, but slowly cleaving, ribozyme thus permitted isolation of enzyme-substrate and enzyme-product complexes without modifying the nucleophile or leaving group of the cleavage reaction, nor any other aspect of the substrate. The predissociation enzyme-product complex structure reveals RNA and metal ion interactions potentially relevant to transition-state stabilization that are absent in precatalytic structures. | ||
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| - | Capturing hammerhead ribozyme structures in action by modulating general base catalysis.,Chi YI, Martick M, Lares M, Kim R, Scott WG, Kim SH PLoS Biol. 2008 Sep 30;6(9):e234. PMID:18834200<ref>PMID:18834200</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2quw" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Ribozyme 3D structures|Ribozyme 3D structures]] | *[[Ribozyme 3D structures|Ribozyme 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Hammerhead Ribozyme G12A mutant after cleavage
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