3bvs
From Proteopedia
(Difference between revisions)
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<StructureSection load='3bvs' size='340' side='right'caption='[[3bvs]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='3bvs' size='340' side='right'caption='[[3bvs]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3bvs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[3bvs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_ATCC_10987 Bacillus cereus ATCC 10987]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BVS FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bvs OCA], [https://pdbe.org/3bvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bvs RCSB], [https://www.ebi.ac.uk/pdbsum/3bvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bvs ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bvs OCA], [https://pdbe.org/3bvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bvs RCSB], [https://www.ebi.ac.uk/pdbsum/3bvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bvs ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q816E8_BACCR Q816E8_BACCR] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bvs ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bvs ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | DNA glycosylases safeguard the genome by locating and excising chemically modified bases from DNA. AlkD is a recently discovered bacterial DNA glycosylase that removes positively charged methylpurines from DNA, and was predicted to adopt a protein fold distinct from from those of other DNA repair proteins. The crystal structure of Bacillus cereus AlkD presented here shows that the protein is composed exclusively of helical HEAT-like repeats, which form a solenoid perfectly shaped to accommodate a DNA duplex on the concave surface. Structural analysis of the variant HEAT repeats in AlkD provides a rationale for how this protein scaffolding motif has been modified to bind DNA. We report 7mG excision and DNA binding activities of AlkD mutants, along with a comparison of alkylpurine DNA glycosylase structures. Together, these data provide important insight into the requirements for alkylation repair within DNA and suggest that AlkD utilizes a novel strategy to manipulate DNA in its search for alkylpurine bases. | ||
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| - | A new protein architecture for processing alkylation damaged DNA: the crystal structure of DNA glycosylase AlkD.,Rubinson EH, Metz AH, O'Quin J, Eichman BF J Mol Biol. 2008 Aug 1;381(1):13-23. Epub 2008 Jun 5. PMID:18585735<ref>PMID:18585735</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 3bvs" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]] | *[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Bacillus cereus]] | + | [[Category: Bacillus cereus ATCC 10987]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Eichman | + | [[Category: Eichman BF]] |
| - | [[Category: Rubinson | + | [[Category: Rubinson EH]] |
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Current revision
Crystal Structure of Bacillus cereus Alkylpurine DNA Glycosylase AlkD
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