3g1z

<table><tr><td colspan='2'>[[3g1z]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G1Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3G1Z FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysine--tRNA_ligase Lysine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.6 6.1.1.6] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3g1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g1z OCA], [http://pdbe.org/3g1z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3g1z RCSB], [http://www.ebi.ac.uk/pdbsum/3g1z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3g1z ProSAT], [http://www.topsan.org/Proteins/CSGID/3g1z TOPSAN]</span></td></tr>

[[http://www.uniprot.org/uniprot/EPMA_SALTY EPMA_SALTY]] With EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF-P) on 'Lys-34'. Catalyzes the ATP-dependent activation of (R)-beta-lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of EF-P 'Lys-34' (Probable). Can also use L-alpha-lysine as a substrate, but probably with lower efficiency. Cannot aminoacylate tRNA(Lys) with lysine.[HAMAP-Rule:MF_00174]<ref>PMID:20670890</ref>

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== Publication Abstract from PubMed ==

We report an interaction between poxA, encoding a paralog of lysyl tRNA-synthetase, and the closely linked yjeK gene, encoding a putative 2,3-beta-lysine aminomutase, that is critical for virulence and stress resistance in Salmonella enterica. Salmonella poxA and yjeK mutants share extensive phenotypic pleiotropy, including attenuated virulence in mice, an increased ability to respire under nutrient-limiting conditions, hypersusceptibility to a variety of diverse growth inhibitors, and altered expression of multiple proteins, including several encoded on the SPI-1 pathogenicity island. PoxA mediates posttranslational modification of bacterial elongation factor P (EF-P), analogous to the modification of the eukaryotic EF-P homolog, eIF5A, with hypusine. The modification of EF-P is a mechanism of regulation whereby PoxA acts as an aminoacyl-tRNA synthetase that attaches an amino acid to a protein resembling tRNA rather than to a tRNA.

PoxA, yjeK, and elongation factor P coordinately modulate virulence and drug resistance in Salmonella enterica.,Navarre WW, Zou SB, Roy H, Xie JL, Savchenko A, Singer A, Edvokimova E, Prost LR, Kumar R, Ibba M, Fang FC Mol Cell. 2010 Jul 30;39(2):209-21. PMID:20670890<ref>PMID:20670890</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 3g1z" style="background-color:#fffaf0;"></div>

[[Category: Lysine--tRNA ligase]]

[[Category: Anderson, W F]]

[[Category: Structural genomic]]

[[Category: Cuff, M E]]

[[Category: Edwards, A M]]

[[Category: Evdokimova, E]]

[[Category: Kudritska, M]]

[[Category: Savchenko, A]]

[[Category: Singer, A U]]

[[Category: T-rna synthetase]]