1qgi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1qgi.gif|left|200px]]
[[Image:1qgi.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1qgi |SIZE=350|CAPTION= <scene name='initialview01'>1qgi</scene>, resolution 1.6&Aring;
+
The line below this paragraph, containing "STRUCTURE_1qgi", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=GCS:D-GLUCOSAMINE'>GCS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitosanase Chitosanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.132 3.2.1.132] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1qgi| PDB=1qgi | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qgi OCA], [http://www.ebi.ac.uk/pdbsum/1qgi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1qgi RCSB]</span>
+
-
}}
+
'''CHITOSANASE FROM BACILLUS CIRCULANS'''
'''CHITOSANASE FROM BACILLUS CIRCULANS'''
Line 32: Line 29:
[[Category: Nagata, Y.]]
[[Category: Nagata, Y.]]
[[Category: Saito, J.]]
[[Category: Saito, J.]]
-
[[Category: chitosan degradation]]
+
[[Category: Chitosan degradation]]
-
[[Category: hydrolase]]
+
[[Category: Hydrolase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:14:26 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:13:11 2008''
+

Revision as of 03:14, 3 May 2008

Image:1qgi.gif

Template:STRUCTURE 1qgi

CHITOSANASE FROM BACILLUS CIRCULANS


Overview

Chitosanase from Bacillus circulans MH-K1 is a 29-kDa extracellular protein composed of 259 amino acids. The crystal structure of chitosanase from B. circulans MH-K1 has been determined by multiwavelength anomalous diffraction method and refined to crystallographic R = 19.2% (R(free) = 23.5%) for the diffraction data at 1.6-A resolution collected by synchrotron radiation. The enzyme has two globular upper and lower domains, which generate the active site cleft for the substrate binding. The overall molecular folding is similar to chitosanase from Streptomyces sp. N174, although there is only 20% identity at the amino acid sequence level between both chitosanases. However, there are three regions in which the topology is remarkably different. In addition, the disulfide bridge between Cys(50) and Cys(124) joins the beta1 strand and the alpha7 helix, which is not conserved among other chitosanases. The orientation of two backbone helices, which connect the two domains, is also different and is responsible for the differences in size and shape of the active site cleft in these two chitosanases. This structural difference in the active site cleft is the reason why the enzymes specifically recognize different substrates and catalyze different types of chitosan degradation.

About this Structure

1QGI is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.

Reference

Crystal structure of chitosanase from Bacillus circulans MH-K1 at 1.6-A resolution and its substrate recognition mechanism., Saito J, Kita A, Higuchi Y, Nagata Y, Ando A, Miki K, J Biol Chem. 1999 Oct 22;274(43):30818-25. PMID:10521473 Page seeded by OCA on Sat May 3 06:14:26 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qgi"
Personal tools