3io0
From Proteopedia
(Difference between revisions)
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<StructureSection load='3io0' size='340' side='right'caption='[[3io0]], [[Resolution|resolution]] 3.00Å' scene=''> | <StructureSection load='3io0' size='340' side='right'caption='[[3io0]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3io0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[3io0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_kluyveri_DSM_555 Clostridium kluyveri DSM 555]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IO0 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3io0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3io0 OCA], [https://pdbe.org/3io0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3io0 RCSB], [https://www.ebi.ac.uk/pdbsum/3io0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3io0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3io0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3io0 OCA], [https://pdbe.org/3io0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3io0 RCSB], [https://www.ebi.ac.uk/pdbsum/3io0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3io0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A5N734_CLOK5 A5N734_CLOK5] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3io0 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3io0 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | It has been suggested that ethanol metabolism in the strict anaerobe Clostridium kluyveri occurs within a metabolosome, a subcellular proteinaceous bacterial microcompartment. Two bacterial microcompartment shell proteins [EtuA (ethanol utilization shell protein A) and EtuB] are found encoded on the genome clustered with the genes for ethanol utilization. The function of the bacterial microcompartment is to facilitate fermentation by sequestering the enzymes, substrates and intermediates. Recent structural studies of bacterial microcompartment proteins have revealed both hexamers and pentamers that assemble to generate the pseudo-icosahedral bacterial microcompartment shell. Some of these shell proteins have pores on their symmetry axes. Here we report the structure of the trimeric bacterial microcompartment protein EtuB, which has a tandem structural repeat within the subunit and pseudo-hexagonal symmetry. The pores in the EtuB trimer are within the subunits rather than between symmetry related subunits. We suggest that the evolutionary advantage of this is that it releases the pore from the rotational symmetry constraint allowing more precise control of the fluxes of asymmetric molecules, such as ethanol, across the pore. We also model EtuA and demonstrate that the two proteins have the potential to interact to generate the casing for a metabolosome. | ||
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| - | Structure of a trimeric bacterial microcompartment shell protein, EtuB, associated with ethanol utilization in Clostridium kluyveri.,Heldt D, Frank S, Seyedarabi A, Ladikis D, Parsons JB, Warren MJ, Pickersgill RW Biochem J. 2009 Sep 25;423(2):199-207. PMID:19635047<ref>PMID:19635047</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 3io0" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Clostridium kluyveri DSM 555]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Pickersgill | + | [[Category: Pickersgill RW]] |
| - | [[Category: Warren | + | [[Category: Warren MJ]] |
| - | + | ||
| - | + | ||
Current revision
Crystal structure of EtuB from Clostridium kluyveri
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