3kl4

<StructureSection load='3kl4' size='340' side='right' caption='[[3kl4]], [[Resolution|resolution]] 3.50&Aring;' scene=''>

<table><tr><td colspan='2'>[[3kl4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KL4 FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ffh|2ffh]], [[1qzx|1qzx]], [[1qzw|1qzw]], [[1dul|1dul]], [[1j8m|1j8m]], [[2j28|2j28]]</td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">srp54, SSO0971 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Signal-recognition-particle_GTPase Signal-recognition-particle GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.4 3.6.5.4] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kl4 OCA], [http://pdbe.org/3kl4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kl4 RCSB], [http://www.ebi.ac.uk/pdbsum/3kl4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kl4 ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/SRP54_SULSO SRP54_SULSO]] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).

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== Publication Abstract from PubMed ==

Targeting of proteins to appropriate subcellular compartments is a crucial process in all living cells. Secretory and membrane proteins usually contain an amino-terminal signal peptide, which is recognized by the signal recognition particle (SRP) when nascent polypeptide chains emerge from the ribosome. The SRP-ribosome nascent chain complex is then targeted through its GTP-dependent interaction with SRP receptor to the protein-conducting channel on endoplasmic reticulum membrane in eukaryotes or plasma membrane in bacteria. A universally conserved component of SRP (refs 1, 2), SRP54 or its bacterial homologue, fifty-four homologue (Ffh), binds the signal peptides, which have a highly divergent sequence divisible into a positively charged n-region, an h-region commonly containing 8-20 hydrophobic residues and a polar c-region. No structure has been reported that exemplifies SRP54 binding of any signal sequence. Here we have produced a fusion protein between Sulfolobus solfataricus SRP54 (Ffh) and a signal peptide connected via a flexible linker. This fusion protein oligomerizes in solution through interaction between the SRP54 and signal peptide moieties belonging to different chains, and it is functional, as demonstrated by its ability to bind SRP RNA and SRP receptor FtsY. We present the crystal structure at 3.5 A resolution of an SRP54-signal peptide complex in the dimer, which reveals how a signal sequence is recognized by SRP54.

Recognition of a signal peptide by the signal recognition particle.,Janda CY, Li J, Oubridge C, Hernandez H, Robinson CV, Nagai K Nature. 2010 May 27;465(7297):507-10. Epub 2010 Apr 4. PMID:20364120<ref>PMID:20364120</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 3kl4" style="background-color:#fffaf0;"></div>

*[[Signal recognition particle protein|Signal recognition particle protein]]

[[Category: Atcc 18824]]

[[Category: Sacs2]]

[[Category: Signal-recognition-particle GTPase]]

[[Category: Janda, C Y]]

[[Category: Li, J]]

[[Category: Nagai, K]]

[[Category: Oubridge, C]]

[[Category: Transmembrane]]