3n1e

<StructureSection load='3n1e' size='340' side='right' caption='[[3n1e]], [[Resolution|resolution]] 1.70&Aring;' scene=''>

<table><tr><td colspan='2'>[[3n1e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N1E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N1E FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a2f|2a2f]], [[2fji|2fji]], [[3fhn|3fhn]], [[2pfv|2pfv]], [[3hr0|3hr0]], [[3n1b|3n1b]]</td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Vps54 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n1e OCA], [http://pdbe.org/3n1e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3n1e RCSB], [http://www.ebi.ac.uk/pdbsum/3n1e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3n1e ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/VPS54_MOUSE VPS54_MOUSE]] Note=Defects in Vps54 are the cause of wobbler phenotype (wr). Wr is autosomal recessive and is a spontaneous mutation discovered almost 50 years ago. It causes spinal muscular atrophy and defective spermiogenesis.<ref>PMID:16244655</ref>

[[http://www.uniprot.org/uniprot/VPS54_MOUSE VPS54_MOUSE]] May be involved in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (By similarity).

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n1/3n1e_consurf.spt"</scriptWhenChecked>

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== Publication Abstract from PubMed ==

The multisubunit Golgi-associated retrograde protein (GARP) complex is required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. Mutation of leucine-967 to glutamine in the Vps54 subunit of GARP is responsible for spinal muscular atrophy in the wobbler mouse, an animal model of amyotrophic lateral sclerosis. The crystal structure at 1.7 A resolution of the mouse Vps54 C-terminal fragment harboring leucine-967, in conjunction with comparative sequence analysis, reveals that Vps54 has a continuous alpha-helical bundle organization similar to that of other multisubunit tethering complexes. The structure shows that leucine-967 is buried within the alpha-helical bundle through predominantly hydrophobic interactions that are critical for domain stability and folding in vitro. Mutation of this residue to glutamine does not prevent integration of Vps54 into the GARP complex but greatly reduces the half-life and levels of the protein in vivo. Severely reduced levels of mutant Vps54 and, consequently, of the whole GARP complex underlie the phenotype of the wobbler mouse.

 

<div class="pdbe-citations 3n1e" style="background-color:#fffaf0;"></div>

[[Category: Lk3 transgenic mice]]

[[Category: Abascal-Palacios, G]]

[[Category: Bonifacino, J S]]

[[Category: Hierro, A]]

[[Category: Kajava, A]]

[[Category: Perez-Victoria, F J]]

[[Category: Pioro, E P]]

[[Category: Tascon, I]]

[[Category: Vesicle trafficking]]