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| | <StructureSection load='3ogz' size='340' side='right'caption='[[3ogz]], [[Resolution|resolution]] 2.03Å' scene=''> | | <StructureSection load='3ogz' size='340' side='right'caption='[[3ogz]], [[Resolution|resolution]] 2.03Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ogz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OGZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ogz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OGZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OGZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3oh0|3oh0]], [[3oh1|3oh1]], [[3oh2|3oh2]], [[3oh3|3oh3]], [[3oh4|3oh4]]</div></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">USP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5664 LEIMA])</td></tr> | + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/UTP-monosaccharide-1-phosphate_uridylyltransferase UTP-monosaccharide-1-phosphate uridylyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.64 2.7.7.64] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ogz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ogz OCA], [https://pdbe.org/3ogz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ogz RCSB], [https://www.ebi.ac.uk/pdbsum/3ogz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ogz ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ogz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ogz OCA], [https://pdbe.org/3ogz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ogz RCSB], [https://www.ebi.ac.uk/pdbsum/3ogz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ogz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/D3G6S4_LEIMA D3G6S4_LEIMA] |
| - | Nucleotide sugars and the enzymes that are responsible for their synthesis are indispensable for the production of complex carbohydrates and, thus, for elaboration of a protective cellular coat for many organisms such as the protozoan parasite Leishmania. These activated sugars are synthesized de novo or derived from salvaged monosaccharides. In addition to UDP-glucose (UDP-Glc) pyrophosphorylase, which catalyzes the formation of UDP-Glc from substrates UTP and glucose-1-phosphate, Leishmania major and plants express a UDP-sugar pyrophosphorylase (USP) that exhibits broad substrate specificity in vitro. The enzyme, likely involved in monosaccharide salvage, preferentially generates UDP-Glc and UDP-galactose, but it may also activate other hexose- or pentose-1-phosphates such as galacturonic acid-1-phosphate or arabinose-1-phosphate. In order to gain insight into structural features governing the differences in substrate specificity, we determined the crystal structure of the L. major USP in the APO-, UTP-, and UDP-sugar-bound conformations. The overall tripartite structure of USP exhibits a significant structural homology to other nucleotidyldiphosphate-glucose pyrophosphorylases. The obtained USP structures reveal the structural rearrangements occurring during the stepwise binding process of the substrates. Moreover, the different product complexes explain the broad substrate specificity of USP, which is enabled by structural changes in the sugar binding region of the active site.
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| - | | + | |
| - | Structural Basis for the Broad Substrate Range of the UDP-Sugar Pyrophosphorylase from Leishmania major.,Dickmanns A, Damerow S, Neumann P, Schulz EC, Lamerz AC, Routier FH, Ficner R J Mol Biol. 2010 Nov 10. PMID:21073876<ref>PMID:21073876</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3ogz" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Leima]] | + | [[Category: Leishmania major]] |
| - | [[Category: UTP-monosaccharide-1-phosphate uridylyltransferase]]
| + | [[Category: Damerow S]] |
| - | [[Category: Damerow, S]] | + | [[Category: Dickmanns A]] |
| - | [[Category: Dickmanns, A]] | + | [[Category: Ficner R]] |
| - | [[Category: Ficner, R]] | + | [[Category: Lamerz A]] |
| - | [[Category: Lamerz, A]] | + | [[Category: Neumann P]] |
| - | [[Category: Neumann, P]] | + | [[Category: Routier F]] |
| - | [[Category: Routier, F]] | + | [[Category: Schulz E-C]] |
| - | [[Category: Schulz, E C]] | + | |
| - | [[Category: Left handed beta helix]]
| + | |
| - | [[Category: Rossmann fold]]
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| - | [[Category: Transferase]]
| + | |
| - | [[Category: Udp sugar pyrophosphorylase]]
| + | |
