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3oo3

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Current revision (10:35, 21 February 2024) (edit) (undo)
 
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<StructureSection load='3oo3' size='340' side='right'caption='[[3oo3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='3oo3' size='340' side='right'caption='[[3oo3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3oo3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinoplanes_teichomyceticus"_parenti_et_al._1978 "actinoplanes teichomyceticus" parenti et al. 1978]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OO3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OO3 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3oo3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinoplanes_teichomyceticus Actinoplanes teichomyceticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OO3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OO3 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Orf6*, tcp19 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1867 "Actinoplanes teichomyceticus" Parenti et al. 1978])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oo3 OCA], [https://pdbe.org/3oo3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oo3 RCSB], [https://www.ebi.ac.uk/pdbsum/3oo3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oo3 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oo3 OCA], [https://pdbe.org/3oo3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oo3 RCSB], [https://www.ebi.ac.uk/pdbsum/3oo3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oo3 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q6ZZI7_ACTTI Q6ZZI7_ACTTI]
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The lipoglycopeptide antibiotic teicoplanin has proven efficacy against gram-positive pathogens. Teicoplanin is distinguished from the vancomycin-type glycopeptide antibiotics, by the presence of an additional cross-link between the aromatic amino acids 1 and 3 that is catalyzed by the cytochrome P450 monooxygenase Orf6* (CYP165D3). As a goal towards understanding the mechanism of this phenol-coupling reaction, we have characterized recombinant Orf6* and determined its crystal structure to 2.2-A resolution. Although the structure of Orf6* reveals the core fold common to other P450 monooxygenases, there are subtle differences in the disposition of secondary structure elements near the active site cavity necessary to accommodate its complex heptapeptide substrate. Specifically, the orientation of the F and G helices in Orf6* results in a more closed active site than found in the vancomycin oxidative enzymes OxyB and OxyC. In addition, Met226 in the I helix replaces the more typical Gly/Ala residue that is positioned above the heme porphyrin ring, where it forms a hydrogen bond with a heme iron-bound water molecule. Sequence comparisons with other phenol-coupling P450 monooxygenases suggest that Met226 plays a role in determining the substrate regiospecificity of Orf6*. These features provide further insights into the mechanism of the cross-linking mechanisms that occur during glycopeptide antibiotics biosynthesis. Proteins 2011; (c) 2011 Wiley-Liss, Inc.
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Crystal structure of a phenol-coupling P450 monooxygenase involved in teicoplanin biosynthesis.,Li Z, Rupasinghe SG, Schuler MA, Nair SK Proteins. 2011 Jan 21. doi: 10.1002/prot.22996. PMID:21445994<ref>PMID:21445994</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3oo3" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Actinoplanes teichomyceticus parenti et al. 1978]]
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[[Category: Actinoplanes teichomyceticus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Li, Z]]
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[[Category: Li Z]]
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[[Category: Nair, S K]]
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[[Category: Nair SK]]
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[[Category: Cytochrome p450]]
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[[Category: Monooxygenase]]
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[[Category: Oxidoreductase]]
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[[Category: Pcd-teicoplanin aglycone]]
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Current revision

Crystal Structure of the Orf6* (CYP165D3) Monooxygenase Involved in Teicoplanin Biosynthesis

PDB ID 3oo3

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