3p7i

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Current revision (10:39, 21 February 2024) (edit) (undo)
 
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<StructureSection load='3p7i' size='340' side='right'caption='[[3p7i]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
<StructureSection load='3p7i' size='340' side='right'caption='[[3p7i]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3p7i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecout Ecout]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P7I FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3p7i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_UTI89 Escherichia coli UTI89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P7I FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P7I:(2-AMINOETHYL)PHOSPHONIC+ACID'>P7I</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">phnD, UTI89_C4699 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=364106 ECOUT])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P7I:(2-AMINOETHYL)PHOSPHONIC+ACID'>P7I</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p7i OCA], [https://pdbe.org/3p7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p7i RCSB], [https://www.ebi.ac.uk/pdbsum/3p7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p7i ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p7i OCA], [https://pdbe.org/3p7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p7i RCSB], [https://www.ebi.ac.uk/pdbsum/3p7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p7i ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q1R3F7_ECOUT Q1R3F7_ECOUT]
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The phnD gene of Escherichia coli encodes the periplasmic binding protein of the phosphonate (Pn) uptake and utilization pathway. We have crystallized and determined structures of E. coli PhnD (EcPhnD) in the absence of ligand and in complex with the environmentally abundant 2-aminoethylphosphonate (2AEP). Similar to other bacterial periplasmic binding proteins, 2AEP binds near the center of mass of EcPhnD in a cleft formed between two lobes. Comparison of the open, unliganded structure with the closed 2AEP-bound structure shows that the two lobes pivot around a hinge by approximately 70 degrees between the two states. Extensive hydrogen bonding and electrostatic interactions stabilize 2AEP, which binds to EcPhnD with low nanomolar affinity. These structures provide insight into Pn uptake by bacteria and facilitated the rational design of high signal-to-noise Pn biosensors based on both coupled small-molecule dyes and autocatalytic fluorescent proteins.
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Structure of the Escherichia coli Phosphonate Binding Protein PhnD and Rationally Optimized Phosphonate Biosensors.,Alicea I, Marvin JS, Miklos AE, Ellington AD, Looger LL, Schreiter ER J Mol Biol. 2011 Dec 2;414(3):356-69. Epub 2011 Oct 12. PMID:22019591<ref>PMID:22019591</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3p7i" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
*[[ABC transporter 3D structures|ABC transporter 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Ecout]]
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[[Category: Escherichia coli UTI89]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Alicea, I]]
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[[Category: Alicea I]]
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[[Category: Schreiter, E R]]
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[[Category: Schreiter ER]]
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[[Category: Phosphonate binding protein]]
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[[Category: Transport protein]]
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Current revision

Crystal structure of Escherichia coli PhnD in complex with 2-aminoethyl phosphonate

PDB ID 3p7i

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