3pm1

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<StructureSection load='3pm1' size='340' side='right'caption='[[3pm1]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='3pm1' size='340' side='right'caption='[[3pm1]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3pm1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PM1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3pm1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PM1 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ET:ETHIDIUM'>ET</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jup|1jup]], [[1jus|1jus]], [[1jty|1jty]], [[1jt6|1jt6]], [[3bqz|3bqz]], [[3br0|3br0]], [[3br1|3br1]], [[3br2|3br2]], [[3br4|3br4]], [[3br5|3br5]], [[3br6|3br6]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ET:ETHIDIUM'>ET</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">qacR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pm1 OCA], [https://pdbe.org/3pm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pm1 RCSB], [https://www.ebi.ac.uk/pdbsum/3pm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pm1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pm1 OCA], [https://pdbe.org/3pm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pm1 RCSB], [https://www.ebi.ac.uk/pdbsum/3pm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pm1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/QACR_STAAM QACR_STAAM]] Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA (By similarity).
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[https://www.uniprot.org/uniprot/QACR_STAAM QACR_STAAM] Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Structures of the multidrug-binding repressor protein QacR with monovalent and bivalent cationic drugs revealed that the carboxylate side-chains of E90 and E120 were proximal to the positively charged nitrogens of the ligands ethidium, malachite green and rhodamine 6G, and therefore may contribute to drug neutralization and binding affinity. Here, we report structural, biochemical and in vivo effects of substituting these glutamate residues. Unexpectedly, substitutions had little impact on ligand affinity or in vivo induction capabilities. Structures of QacR(E90Q) and QacR(E120Q) with ethidium or malachite green took similar global conformations that differed significantly from all previously described QacR-drug complexes but still prohibited binding to cognate DNA. Strikingly, the QacR(E90Q)-rhodamine 6G complex revealed two mutually exclusive rhodamine 6G binding sites. Despite multiple structural changes, all drug binding was essentially isoenergetic. Thus, these data strongly suggest that rather than contributing significantly to ligand binding affinity, the role of acidic residues lining the QacR multidrug-binding pocket is primarily to attract and guide cationic drugs to the "best available" positions within the pocket that elicit QacR induction.
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A single acidic residue can guide binding site selection but does not govern QacR cationic-drug affinity.,Peters KM, Brooks BE, Schumacher MA, Skurray RA, Brennan RG, Brown MH PLoS One. 2011 Jan 17;6(1):e15974. PMID:21264225<ref>PMID:21264225</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3pm1" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
*[[Tetracycline repressor protein 3D structures|Tetracycline repressor protein 3D structures]]
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Staam]]
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[[Category: Staphylococcus aureus subsp. aureus Mu50]]
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[[Category: Schumacher, M A]]
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[[Category: Schumacher MA]]
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[[Category: Dna]]
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[[Category: Multidrug resistance]]
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[[Category: Nucleoid]]
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[[Category: Tetr family member]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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Current revision

Structure of QacR E90Q bound to Ethidium

PDB ID 3pm1

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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