3q8d

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<StructureSection load='3q8d' size='340' side='right'caption='[[3q8d]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='3q8d' size='340' side='right'caption='[[3q8d]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3q8d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q8D FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3q8d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q8D FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CPS:3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE'>CPS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">recO ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), ssb ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CPS:3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE'>CPS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q8d OCA], [https://pdbe.org/3q8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q8d RCSB], [https://www.ebi.ac.uk/pdbsum/3q8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q8d ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q8d OCA], [https://pdbe.org/3q8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q8d RCSB], [https://www.ebi.ac.uk/pdbsum/3q8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q8d ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/D5CXR1_ECOKI D5CXR1_ECOKI]] Involved in DNA repair and RecF pathway recombination.[HAMAP-Rule:MF_00201][SAAS:SAAS00018757]
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[https://www.uniprot.org/uniprot/RECO_ECOLI RECO_ECOLI] Involved in DNA repair and RecF pathway recombination.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RecO is a recombination mediator protein (RMP) important for homologous recombination, replication repair and DNA annealing in bacteria. In all pathways, the single-stranded (ss) DNA binding protein, SSB, plays an inhibitory role by protecting ssDNA from annealing and recombinase binding. Conversely, SSB may stimulate each reaction through direct interaction with RecO. We present a crystal structure of Escherichia coli RecO bound to the conserved SSB C-terminus (SSB-Ct). SSB-Ct binds the hydrophobic pocket of RecO in a conformation similar to that observed in the ExoI/SSB-Ct complex. Hydrophobic interactions facilitate binding of SSB-Ct to RecO and RecO/RecR complex in both low and moderate ionic strength solutions. In contrast, RecO interaction with DNA is inhibited by an elevated salt concentration. The SSB mutant lacking SSB-Ct also inhibits RecO-mediated DNA annealing activity in a salt-dependent manner. Neither RecO nor RecOR dissociates SSB from ssDNA. Therefore, in E. coli, SSB recruits RMPs to ssDNA through SSB-Ct, and RMPs are likely to alter the conformation of SSB-bound ssDNA without SSB dissociation to initiate annealing or recombination. Intriguingly, Deinococcus radiodurans RecO does not bind SSB-Ct and weakly interacts with the peptide in the presence of RecR, suggesting the diverse mechanisms of DNA repair pathways mediated by RecO in different organisms.
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Mechanism of RecO recruitment to DNA by single-stranded DNA binding protein.,Ryzhikov M, Koroleva O, Postnov D, Tran A, Korolev S Nucleic Acids Res. 2011 Apr 18. PMID:21504984<ref>PMID:21504984</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3q8d" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus coli migula 1895]]
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[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Korolev, S]]
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[[Category: Korolev S]]
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[[Category: Koroleva, O]]
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[[Category: Koroleva O]]
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[[Category: Ryzhikov, M]]
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[[Category: Ryzhikov M]]
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[[Category: Dna]]
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[[Category: Dna binding protein]]
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[[Category: Dna-binding protein]]
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[[Category: Ob-fold]]
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[[Category: Recombination initation]]
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[[Category: Recombination initiation]]
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[[Category: Recr]]
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[[Category: Ssb]]
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Current revision

E. coli RecO complex with SSB C-terminus

PDB ID 3q8d

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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