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| | <StructureSection load='3qh6' size='340' side='right'caption='[[3qh6]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='3qh6' size='340' side='right'caption='[[3qh6]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3qh6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlt2 Chlt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QH6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3qh6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis_434/Bu Chlamydia trachomatis 434/Bu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QH6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qh7|3qh7]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTL0548 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=471472 CHLT2])</td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qh6 OCA], [https://pdbe.org/3qh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qh6 RCSB], [https://www.ebi.ac.uk/pdbsum/3qh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qh6 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qh6 OCA], [https://pdbe.org/3qh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qh6 RCSB], [https://www.ebi.ac.uk/pdbsum/3qh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qh6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A0H3MBY2_CHLT2 A0A0H3MBY2_CHLT2] |
| - | Chlamydia trachomatis is a medically important pathogen that encodes a relatively high percentage of proteins with unknown function. The three-dimensional structure of a protein can be very informative regarding the protein's functional characteristics; however, determining protein structures experimentally can be very challenging. Computational methods that model protein structures with sufficient accuracy to facilitate functional studies have had notable successes. To evaluate the accuracy and potential impact of computational protein structure modeling of hypothetical proteins encoded by Chlamydia, a successful computational method termed I-TASSER was utilized to model the three-dimensional structure of a hypothetical protein encoded by open reading frame (ORF) CT296. CT296 has been reported to exhibit functional properties of a divalent cation transcription repressor (DcrA), with similarity to the Escherichia coli iron-responsive transcriptional repressor, Fur. Unexpectedly, the I-TASSER model of CT296 exhibited no structural similarity to any DNA-interacting proteins or motifs. To validate the I-TASSER-generated model, the structure of CT296 was solved experimentally using X-ray crystallography. Impressively, the ab initio I-TASSER-generated model closely matched (2.72-A C(alpha) root mean square deviation [RMSD]) the high-resolution (1.8-A) crystal structure of CT296. Modeled and experimentally determined structures of CT296 share structural characteristics of non-heme Fe(II) 2-oxoglutarate-dependent enzymes, although key enzymatic residues are not conserved, suggesting a unique biochemical process is likely associated with CT296 function. Additionally, functional analyses did not support prior reports that CT296 has properties shared with divalent cation repressors such as Fur.
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| - | | + | |
| - | Ab initio structural modeling of and experimental validation for Chlamydia trachomatis protein CT296 reveal structural similarity to Fe(II) 2-oxoglutarate-dependent enzymes.,Kemege KE, Hickey JM, Lovell S, Battaile KP, Zhang Y, Hefty PS J Bacteriol. 2011 Dec;193(23):6517-28. Epub 2011 Sep 30. PMID:21965559<ref>PMID:21965559</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3qh6" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chlt2]] | + | [[Category: Chlamydia trachomatis 434/Bu]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Battaile, K P]] | + | [[Category: Battaile KP]] |
| - | [[Category: Hefty, P S]] | + | [[Category: Hefty PS]] |
| - | [[Category: Hickey, J]] | + | [[Category: Hickey J]] |
| - | [[Category: Kemege, K]] | + | [[Category: Kemege K]] |
| - | [[Category: Lovell, S]] | + | [[Category: Lovell S]] |
| - | [[Category: Zhang, Y]] | + | [[Category: Zhang Y]] |
| - | [[Category: Chlamydia]]
| + | |
| - | [[Category: Ct296]]
| + | |
| - | [[Category: Iron]]
| + | |
| - | [[Category: Modeling]]
| + | |
| - | [[Category: Unknown function]]
| + | |
