3r4v

Structural highlights

Function

PHUZ_BP201 A tubulin-like GTPase that forms filaments, which are required for positioning viral DNA and capsids in the middle of the host cell for optimal replication (PubMed:28082593, PubMed:22726436, PubMed:25429514, PubMed:31199917, PubMed:28813669). The motor component of a partition system which pushes phage DNA (encased by protein gp105) to the center of the bacterial host cell (PubMed:28082593, PubMed:28813669). Also required for movement of phage capsids to the vicinity of the viral DNA and rotation of the encased viral DNA at midcell (PubMed:31199917). Forms filaments during the lytic phase, which position phage DNA at the center of the bacterial host cell (PubMed:22726436, PubMed:25429514, PubMed:31199917, PubMed:28082593, PubMed:28813669). Filaments have a three-stranded intertwined achitecture and form a spindle-like cytoskeleton within the infected cell (PubMed:24631461). Has GTPase activity (PubMed:22726436). Filaments grow at the plus end and depolymerize at the minus end, a process called treadmilling, and switch from growing in a polar manner to catastrophic depolymerization, i.e. they display dynamic instability, like tubulin. In infected host cells the filament ends close to the cell pole are relatively stable, while the other end near the phage DNA is highly dynamic (PubMed:25429514). Both capsid movement and DNA rotation probably require treadmilling (Probable).^{[1] [2] [3] [4] [5] [6] [7]}

References

1. ↑ Kraemer JA, Erb ML, Waddling CA, Montabana EA, Zehr EA, Wang H, Nguyen K, Pham DS, Agard DA, Pogliano J. A phage tubulin assembles dynamic filaments by an atypical mechanism to center viral DNA within the host cell. Cell. 2012 Jun 22;149(7):1488-99. doi: 10.1016/j.cell.2012.04.034. PMID:22726436 doi:http://dx.doi.org/10.1016/j.cell.2012.04.034
2. ↑ Zehr EA, Kraemer JA, Erb ML, Coker JK, Montabana EA, Pogliano J, Agard DA. The Structure and Assembly Mechanism of a Novel Three-Stranded Tubulin Filament that Centers Phage DNA. Structure. 2014 Mar 11. pii: S0969-2126(14)00046-X. doi:, 10.1016/j.str.2014.02.006. PMID:24631461 doi:http://dx.doi.org/10.1016/j.str.2014.02.006
3. ↑ Erb ML, Kraemer JA, Coker JK, Chaikeeratisak V, Nonejuie P, Agard DA, Pogliano J. A bacteriophage tubulin harnesses dynamic instability to center DNA in infected cells. Elife. 2014 Nov 27;3:e03197. PMID:25429514 doi:10.7554/eLife.03197
4. ↑ Chaikeeratisak V, Nguyen K, Khanna K, Brilot AF, Erb ML, Coker JK, Vavilina A, Newton GL, Buschauer R, Pogliano K, Villa E, Agard DA, Pogliano J. Assembly of a nucleus-like structure during viral replication in bacteria. Science. 2017 Jan 13;355(6321):194-197. doi: 10.1126/science.aal2130. PMID:28082593 doi:http://dx.doi.org/10.1126/science.aal2130
5. ↑ Chaikeeratisak V, Nguyen K, Egan ME, Erb ML, Vavilina A, Pogliano J. The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages. Cell Rep. 2017 Aug 15;20(7):1563-1571. doi: 10.1016/j.celrep.2017.07.064. PMID:28813669 doi:http://dx.doi.org/10.1016/j.celrep.2017.07.064
6. ↑ Chaikeeratisak V, Khanna K, Nguyen KT, Sugie J, Egan ME, Erb ML, Vavilina A, Nonejuie P, Nieweglowska E, Pogliano K, Agard DA, Villa E, Pogliano J. Viral Capsid Trafficking along Treadmilling Tubulin Filaments in Bacteria. Cell. 2019 Jun 13;177(7):1771-1780.e12. PMID:31199917 doi:10.1016/j.cell.2019.05.032
7. ↑ Chaikeeratisak V, Khanna K, Nguyen KT, Sugie J, Egan ME, Erb ML, Vavilina A, Nonejuie P, Nieweglowska E, Pogliano K, Agard DA, Villa E, Pogliano J. Viral Capsid Trafficking along Treadmilling Tubulin Filaments in Bacteria. Cell. 2019 Jun 13;177(7):1771-1780.e12. PMID:31199917 doi:10.1016/j.cell.2019.05.032