This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7tn8
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of Zea mays Inositol-tetrakisphosphate Kinase 1 mutant (ZmITPK1-H192A) in complex with InsP6== | ==Crystal structure of Zea mays Inositol-tetrakisphosphate Kinase 1 mutant (ZmITPK1-H192A) in complex with InsP6== | ||
| - | <StructureSection load='7tn8' size='340' side='right'caption='[[7tn8]]' scene=''> | + | <StructureSection load='7tn8' size='340' side='right'caption='[[7tn8]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TN8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7tn8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TN8 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tn8 OCA], [https://pdbe.org/7tn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tn8 RCSB], [https://www.ebi.ac.uk/pdbsum/7tn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tn8 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tn8 OCA], [https://pdbe.org/7tn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tn8 RCSB], [https://www.ebi.ac.uk/pdbsum/7tn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tn8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ITPK1_MAIZE ITPK1_MAIZE] Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3 and participates in phytic acid biosynthesis in developing seeds (PubMed:12586875, PubMed:32123645). Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not (PubMed:12586875). Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway (PubMed:12586875). Also able to phosphorylate Ins(3,5,6)P3 but not Ins(1,4,5)P3, Ins(2,4,5)P3, Ins(1,3,4,6)P4 nor Ins(1,3,5,6)P4. Has higher specific activity on Ins(3,4,5,6)P4 than Ins(1,3,4)P3 and Ins(3,5,6)P3 (PubMed:12586875). Can also could use Ins(1,2,5,6)P4 as a substrate (PubMed:12586875). Able to add a beta-phosphate to the 3 positions of Ins(1,2,3,4,5)P5 and to add beta-phosphate to InsP6 to yield 5-InsP7, thus exhibiting InsP6 kinase activity (PubMed:35635723). Has also Ins(1,3,4,5,6)P5 phosphatase activity (PubMed:35635723).<ref>PMID:12586875</ref> <ref>PMID:32123645</ref> <ref>PMID:35635723</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Zea mays]] | ||
[[Category: Shears SB]] | [[Category: Shears SB]] | ||
[[Category: Wang H]] | [[Category: Wang H]] | ||
[[Category: Zong G]] | [[Category: Zong G]] | ||
Current revision
Crystal structure of Zea mays Inositol-tetrakisphosphate Kinase 1 mutant (ZmITPK1-H192A) in complex with InsP6
| |||||||||||
Categories: Large Structures | Zea mays | Shears SB | Wang H | Zong G
