3c2j
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3c2j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C2J FirstGlance]. <br> | <table><tr><td colspan='2'>[[3c2j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C2J FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GGT:TRIOXACARCIN+A'>GGT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GGT:TRIOXACARCIN+A'>GGT</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c2j OCA], [https://pdbe.org/3c2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c2j RCSB], [https://www.ebi.ac.uk/pdbsum/3c2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c2j ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c2j OCA], [https://pdbe.org/3c2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c2j RCSB], [https://www.ebi.ac.uk/pdbsum/3c2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c2j ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | We report a crystal structure that shows an antibiotic that extracts a nucleobase from a DNA molecule 'caught in the act' after forming a covalent bond but before departing with the base. The structure of trioxacarcin A covalently bound to double-stranded d(AACCGGTT) was determined to 1.78 A resolution by MAD phasing employing brominated oligonucleotides. The DNA-drug complex has a unique structure that combines alkylation (at the N7 position of a guanine), intercalation (on the 3'-side of the alkylated guanine), and base flip-out. An antibiotic-induced flipping-out of a single, nonterminal nucleobase from a DNA duplex was observed for the first time in a crystal structure. | ||
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| - | Crystal structure of trioxacarcin A covalently bound to DNA.,Pfoh R, Laatsch H, Sheldrick GM Nucleic Acids Res. 2008 May 3;. PMID:18453630<ref>PMID:18453630</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 3c2j" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Pfoh | + | [[Category: Pfoh R]] |
| - | [[Category: Sheldrick | + | [[Category: Sheldrick GM]] |
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Current revision
Crystal structure analysis of trioxacarcin A covalently bound to d(AACCGGTT)
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